Detailed information of TA system TA089181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3910306..3910943
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q7N1I2
• Locus tag: PluDJC_RS17295
• Protein ID: WP_011147672.1
• Coordinates: 3910770..3910943 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PluDJC_RS17290
• Protein ID: WP_109791736.1
• Coordinates: 3910306..3910716 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS17250	3906667..3907014	+	348	WP_011147559.1	outer membrane protein assembly factor BamE	-
PluDJC_RS17255	3907070..3907363	-	294	WP_011147560.1	RnfH family protein	-
PluDJC_RS17260	3907356..3907790	-	435	WP_011147561.1	type II toxin-antitoxin system RatA family toxin	-
PluDJC_RS17265	3907947..3908429	+	483	WP_011147562.1	SsrA-binding protein SmpB	-
PluDJC_RS26380	3909183..3909398	-	216	WP_049789790.1	hypothetical protein	-
PluDJC_RS17280	3909462..3909692	+	231	WP_011147669.1	hypothetical protein	-
PluDJC_RS17285	3909689..3909958	+	270	WP_011147670.1	helix-turn-helix domain-containing protein	-
PluDJC_RS17290	3910306..3910716	-	411	WP_109791736.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PluDJC_RS17295	3910770..3910943	-	174	WP_011147672.1	type II toxin-antitoxin system HicA family toxin	Toxin
PluDJC_RS17300	3911063..3912712	-	1650	WP_114599666.1	hypothetical protein	-
PluDJC_RS17305	3912712..3913248	-	537	WP_041380260.1	hypothetical protein	-
PluDJC_RS17310	3913220..3913828	-	609	WP_113049657.1	hypothetical protein	-
PluDJC_RS17315	3913948..3914564	-	617	Protein_3319	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3909689..3919974	10285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-62)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6680.79 Da
• Isoelectric Point: 11.3427

>T89181 WP_011147672.1 NZ_CP024900:c3910943-3910770 [Photorhabdus laumondii subsp. laumondii]
VKQSEFRRWLEAQGVEVSNGTNHLKLRYQGKRSIMPRHPSQEIKEPLRKAIIKQLGL

Nucleotide

• Length: 174 bp

>T89181 NZ_CP024900:c3910943-3910770 [Photorhabdus laumondii subsp. laumondii]
GTGAAGCAAAGCGAGTTTAGACGGTGGCTGGAAGCTCAAGGCGTCGAAGTTTCAAATGGGACAAACCATCTGAAACTGAG
ATACCAAGGGAAACGAAGCATTATGCCGAGACACCCTAGCCAAGAGATCAAAGAGCCGCTGAGGAAAGCAATCATTAAGC
AATTAGGATTGTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15103.26 Da
• Isoelectric Point: 4.2783

>AT89181 WP_109791736.1 NZ_CP024900:c3910716-3910306 [Photorhabdus laumondii subsp. laumondii]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGDTREEALEMALDALITAFEFYFEDNEKIPLPGPAGQDYVDVPLSVASKVLM
LNAFLDSKLTQIELANRMGVKKQEVTRIFDLRHSTKIDTVGKVASAIGHQLTLSIE

Nucleotide

• Length: 411 bp

>AT89181 NZ_CP024900:c3910716-3910306 [Photorhabdus laumondii subsp. laumondii]
ATGCGATATCCAGTCACACTAGAGCCAGTAGAAGAGGGCGGTTATTTCGTCTCATTCCCGGATATCCCCGAAGCTTTAAC
CCAGGGGGATACACGGGAAGAGGCGCTAGAGATGGCACTCGATGCGCTAATAACCGCTTTTGAATTTTACTTCGAAGATA
ATGAAAAAATCCCGCTTCCTGGCCCCGCAGGGCAGGATTATGTTGATGTGCCCTTAAGCGTGGCCTCTAAGGTGCTTATG
CTCAATGCGTTTCTTGATTCCAAGTTAACGCAAATTGAATTAGCCAACCGTATGGGGGTGAAAAAGCAGGAAGTGACCCG
TATCTTTGATTTACGGCACTCAACTAAAATTGATACGGTGGGTAAGGTCGCATCGGCTATCGGACATCAGTTAACTTTGT
CCATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A6L9JNW7