Detailed information of TA system TA089179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3469013..3469668
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q7N2L9
• Locus tag: PluDJC_RS15665
• Protein ID: WP_011147274.1
• Coordinates: 3469013..3469432 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q7N2L8
• Locus tag: PluDJC_RS15670
• Protein ID: WP_011147275.1
• Coordinates: 3469429..3469668 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS15640	3464031..3466322	+	2292	WP_011147269.1	ribonucleoside-diphosphate reductase subunit alpha	-
PluDJC_RS15645	3466335..3467465	+	1131	WP_011147270.1	ribonucleotide-diphosphate reductase subunit beta	-
PluDJC_RS15650	3467465..3467731	+	267	WP_011147271.1	2Fe-2S ferredoxin-like protein	-
PluDJC_RS15655	3467853..3468233	-	381	WP_011147272.1	type II toxin-antitoxin system YafO family toxin	-
PluDJC_RS15660	3468233..3468853	-	621	WP_041380171.1	hypothetical protein	-
PluDJC_RS15665	3469013..3469432	-	420	WP_011147274.1	type II toxin-antitoxin system VapC family toxin	Toxin
PluDJC_RS15670	3469429..3469668	-	240	WP_011147275.1	Arc family DNA-binding protein	Antitoxin
PluDJC_RS15675	3469998..3471011	+	1014	WP_041380173.1	Gfo/Idh/MocA family oxidoreductase	-
PluDJC_RS15680	3471131..3472747	-	1617	WP_011144714.1	IS1634-like element ISPlu4 family transposase	-
PluDJC_RS15685	3473027..3473533	-	507	WP_011147276.1	CdiI family contact-dependent growth inhibition immunity protein	-
PluDJC_RS15690	3473697..3474203	-	507	WP_011147277.1	CdiI family contact-dependent growth inhibition immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3471131..3472747	1616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14785.05 Da
• Isoelectric Point: 6.4726

>T89179 WP_011147274.1 NZ_CP024900:c3469432-3469013 [Photorhabdus laumondii subsp. laumondii]
MILLDTNVVSEPLRLSGHPAVVAWIDEQNVETLYLAAISLAELRFGVAALSNGKRKDTLHRNLERSVVPLFTGRILPFDA
AASEAYAEIMARAKSEGKAIGKADGYIAAIAAANGLIVATRDISPFEAAGLTVINPWKG

Nucleotide

• Length: 420 bp

>T89179 NZ_CP024900:c3469432-3469013 [Photorhabdus laumondii subsp. laumondii]
ATGATACTGCTAGATACGAACGTGGTTTCCGAGCCACTGCGGCTCAGCGGCCACCCCGCTGTGGTTGCCTGGATAGATGA
ACAGAATGTCGAGACGCTTTACTTGGCAGCGATCAGCTTGGCTGAGCTTCGCTTTGGGGTTGCAGCGCTGTCCAATGGTA
AGCGCAAAGATACCCTGCATAGAAACCTAGAGCGGAGTGTTGTTCCTTTATTTACCGGTCGTATCTTGCCATTCGATGCG
GCCGCTTCGGAAGCTTATGCCGAGATCATGGCGCGAGCGAAGAGCGAGGGGAAAGCCATTGGGAAGGCTGACGGGTATAT
TGCGGCTATTGCAGCGGCGAATGGGCTGATTGTTGCAACCCGCGACATATCACCTTTTGAAGCTGCCGGGCTTACTGTTA
TCAACCCTTGGAAGGGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8776.01 Da
• Isoelectric Point: 5.7268

>AT89179 WP_011147275.1 NZ_CP024900:c3469668-3469429 [Photorhabdus laumondii subsp. laumondii]
MPSITVRNVPDEVHRALRVRAATHGRSAEAEIRFILENAVQPEGRIKLGSLLAKIGREAEGVDLEIERDKTPAEPMSFE

Nucleotide

• Length: 240 bp

>AT89179 NZ_CP024900:c3469668-3469429 [Photorhabdus laumondii subsp. laumondii]
ATGCCATCCATTACTGTCAGAAATGTACCGGACGAAGTGCATCGTGCCTTGCGGGTACGCGCAGCCACACATGGCCGTAG
TGCTGAGGCCGAAATCCGGTTTATTCTGGAAAACGCCGTACAACCGGAGGGGCGCATCAAGCTGGGTTCCTTGCTGGCAA
AAATCGGCCGCGAGGCGGAGGGCGTCGATCTTGAGATCGAGCGTGATAAGACACCTGCTGAGCCGATGAGTTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A6L9JKK2

Antitoxin

• AlphaFold DB: A0A6L9JKY2