Detailed information of TA system TA089175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 2674562..2675139
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q7N4L8
• Locus tag: PluDJC_RS12290
• Protein ID: WP_011146555.1
• Coordinates: 2674837..2675139 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PluDJC_RS12285
• Protein ID: WP_011146554.1
• Coordinates: 2674562..2674840 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS12245	2670297..2670830	-	534	WP_113049476.1	type VI secretion system contractile sheath small subunit	-
PluDJC_RS12250	2671555..2671809	+	255	WP_011146550.1	hypothetical protein	-
PluDJC_RS12255	2672054..2672929	-	876	WP_011146551.1	phage tail protein	-
PluDJC_RS12265	2673574..2673846	+	273	WP_011146552.1	CopG family ribbon-helix-helix protein	-
PluDJC_RS12270	2673834..2674124	+	291	WP_011146553.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PluDJC_RS12280	2674349..2674525	+	177	Protein_2345	IS1595 family transposase	-
PluDJC_RS12285	2674562..2674840	-	279	WP_011146554.1	putative addiction module antidote protein	Antitoxin
PluDJC_RS12290	2674837..2675139	-	303	WP_011146555.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PluDJC_RS12295	2675237..2675377	+	141	WP_082302859.1	recombinase family protein	-
PluDJC_RS26545	2675370..2675558	-	189	Protein_2349	helix-turn-helix domain-containing protein	-
PluDJC_RS12305	2675548..2675637	-	90	Protein_2350	class A beta-lactamase	-
PluDJC_RS12310	2676332..2678185	+	1854	WP_011146556.1	hypothetical protein	-
PluDJC_RS12315	2678416..2679387	-	972	WP_011146557.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2674286..2674528	242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-87)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11303.07 Da
• Isoelectric Point: 9.8100

>T89175 WP_011146555.1 NZ_CP024900:c2675139-2674837 [Photorhabdus laumondii subsp. laumondii]
MITVLSTETFDNWIDALKDIRVKTKIQARIRRLKNGNSGDIEPVGEGFSEMRIHEGKGYRVYLKNHGKVIVVLLCGGDKS
TQQKDIARAKAIYKEIEGEL

Nucleotide

• Length: 303 bp

>T89175 NZ_CP024900:c2675139-2674837 [Photorhabdus laumondii subsp. laumondii]
ATGATAACTGTATTAAGTACAGAAACATTTGATAACTGGATTGATGCCCTGAAAGACATCAGGGTAAAAACAAAGATTCA
GGCACGAATTCGCAGACTGAAAAACGGTAATTCTGGCGATATAGAGCCAGTAGGGGAAGGGTTTTCAGAAATGCGGATTC
ATGAAGGTAAAGGCTACAGGGTTTATCTAAAAAATCACGGAAAAGTAATCGTTGTTTTGTTGTGTGGGGGAGATAAAAGT
ACACAGCAAAAAGATATAGCCAGAGCAAAAGCCATTTACAAAGAGATTGAGGGGGAGCTATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10203.76 Da
• Isoelectric Point: 5.6105

>AT89175 WP_011146554.1 NZ_CP024900:c2674840-2674562 [Photorhabdus laumondii subsp. laumondii]
MKLKEYDIAEHLNSEEEMQMYLNEIMEDGDPALVLSALGDIARARNMSQLSREVGMSREGLYHALSGKGNPTFSAMMKIT
KALGLKLHFTGA

Nucleotide

• Length: 279 bp

>AT89175 NZ_CP024900:c2674840-2674562 [Photorhabdus laumondii subsp. laumondii]
ATGAAACTGAAAGAATATGATATTGCAGAACACTTAAACAGTGAAGAAGAAATGCAAATGTATCTTAATGAAATTATGGA
AGATGGTGATCCGGCATTGGTACTGTCTGCGCTGGGTGATATTGCCAGAGCCAGAAACATGAGCCAGCTTTCAAGAGAGG
TCGGTATGTCCAGAGAAGGGCTATATCATGCTCTTTCAGGAAAAGGTAATCCAACATTCAGCGCTATGATGAAAATAACA
AAAGCGTTAGGGCTGAAACTTCATTTCACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6L9JRW2