Detailed information of TA system TA089174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2673574..2674124
• Replicon: chromosome
• Accession: NZ_CP024900
• Organism: Photorhabdus laumondii subsp. laumondii strain DJC

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q7N4M0
• Locus tag: PluDJC_RS12270
• Protein ID: WP_011146553.1
• Coordinates: 2673834..2674124 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q7N4M1
• Locus tag: PluDJC_RS12265
• Protein ID: WP_011146552.1
• Coordinates: 2673574..2673846 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PluDJC_RS12240	2668808..2670289	-	1482	WP_011146548.1	type VI secretion system contractile sheath large subunit	-
PluDJC_RS12245	2670297..2670830	-	534	WP_113049476.1	type VI secretion system contractile sheath small subunit	-
PluDJC_RS12250	2671555..2671809	+	255	WP_011146550.1	hypothetical protein	-
PluDJC_RS12255	2672054..2672929	-	876	WP_011146551.1	phage tail protein	-
PluDJC_RS12265	2673574..2673846	+	273	WP_011146552.1	CopG family ribbon-helix-helix protein	Antitoxin
PluDJC_RS12270	2673834..2674124	+	291	WP_011146553.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PluDJC_RS12280	2674349..2674525	+	177	Protein_2345	IS1595 family transposase	-
PluDJC_RS12285	2674562..2674840	-	279	WP_011146554.1	putative addiction module antidote protein	-
PluDJC_RS12290	2674837..2675139	-	303	WP_011146555.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PluDJC_RS12295	2675237..2675377	+	141	WP_082302859.1	recombinase family protein	-
PluDJC_RS26545	2675370..2675558	-	189	Protein_2349	helix-turn-helix domain-containing protein	-
PluDJC_RS12305	2675548..2675637	-	90	Protein_2350	class A beta-lactamase	-
PluDJC_RS12310	2676332..2678185	+	1854	WP_011146556.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2674286..2674528	242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10867.56 Da
• Isoelectric Point: 10.0918

>T89174 WP_011146553.1 NZ_CP024900:2673834-2674124 [Photorhabdus laumondii subsp. laumondii]
MPRLIWSPPALADIQRLYGFLAKKDKDTARRAIKTIRAGVKMLARQPEAGRPADDMDPAFREWLIDFGSSGYIALYRIEG
ETATIFAVRHQKEAGY

Nucleotide

• Length: 291 bp

>T89174 NZ_CP024900:2673834-2674124 [Photorhabdus laumondii subsp. laumondii]
ATGCCACGTTTGATTTGGTCTCCGCCTGCTCTCGCTGATATACAGCGGCTCTATGGTTTTCTTGCTAAAAAAGACAAAGA
CACTGCTCGTCGAGCTATTAAAACAATACGTGCTGGCGTGAAAATGCTGGCACGTCAGCCTGAAGCAGGACGCCCTGCTG
ACGATATGGATCCGGCATTCCGAGAATGGCTTATTGATTTTGGAAGTAGTGGCTATATCGCCTTGTATCGGATTGAGGGT
GAAACAGCAACAATTTTTGCTGTACGGCATCAAAAAGAGGCCGGATATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10654.87 Da
• Isoelectric Point: 4.6704

>AT89174 WP_011146552.1 NZ_CP024900:2673574-2673846 [Photorhabdus laumondii subsp. laumondii]
MATSVKLDDNLKSRIQYLAEVRHRSAHWIMREAIRDYVEREEKRESFKQDALRAWEAYQENGLHLTLEEADDWLAKLEAG
EDAELPECHV

Nucleotide

• Length: 273 bp

>AT89174 NZ_CP024900:2673574-2673846 [Photorhabdus laumondii subsp. laumondii]
ATGGCAACCTCAGTAAAACTTGACGATAACTTGAAAAGCCGGATCCAGTATTTGGCAGAAGTGCGACATCGTTCAGCTCA
TTGGATAATGCGTGAAGCTATACGTGACTATGTAGAGCGCGAGGAAAAGCGTGAATCTTTCAAACAGGATGCATTGCGTG
CTTGGGAAGCGTATCAAGAGAATGGACTACACCTGACGCTTGAAGAAGCGGACGACTGGTTAGCTAAGCTCGAAGCAGGA
GAAGATGCGGAGTTACCTGAATGCCACGTTTGA

Structures

Toxin

• AlphaFold DB: A0A6L9JTC2

Antitoxin

• AlphaFold DB: A0A6L9JNK5