Detailed information of TA system TA089154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3808892..3809457
• Replicon: chromosome
• Accession: NZ_CP024890
• Organism: Rhodococcus ruber strain YYL

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A098BGQ9
• Locus tag: CSW53_RS17475
• Protein ID: WP_040270293.1
• Coordinates: 3808892..3809248 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8D4K486
• Locus tag: CSW53_RS17480
• Protein ID: WP_040270291.1
• Coordinates: 3809245..3809457 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSW53_RS17455	3805980..3807038	-	1059	WP_102031466.1	beta-mannosidase	-
CSW53_RS17460	3807115..3807645	-	531	WP_017681490.1	pyridoxamine 5'-phosphate oxidase family protein	-
CSW53_RS17470	3807990..3808817	+	828	WP_102031468.1	hypothetical protein	-
CSW53_RS17475	3808892..3809248	-	357	WP_040270293.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSW53_RS17480	3809245..3809457	-	213	WP_040270291.1	ribbon-helix-helix protein, CopG family	Antitoxin
CSW53_RS17485	3809667..3810248	+	582	WP_102031469.1	GNAT family N-acetyltransferase	-
CSW53_RS17490	3810256..3811287	-	1032	WP_102031470.1	hypothetical protein	-
CSW53_RS17495	3811401..3812375	-	975	WP_102031471.1	helix-turn-helix domain-containing protein	-
CSW53_RS17500	3812513..3813151	+	639	WP_048774002.1	isochorismatase family protein	-
CSW53_RS17505	3813288..3814199	+	912	WP_017679961.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3795881..3809457	13576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12780.56 Da
• Isoelectric Point: 7.3225

>T89154 WP_040270293.1 NZ_CP024890:c3809248-3808892 [Rhodococcus ruber]
MIRRAQIHWVDLDPAQGGEAAERRPAVVVSNDRANATAENLGRGVVTVVPLIASTSHVFPFQVLVEPDESGLTRASKAQA
EQVRSVSVRRLDAEPVGKLGVRTLAALDEALRLHLDLR

Nucleotide

• Length: 357 bp

>T89154 NZ_CP024890:c3809248-3808892 [Rhodococcus ruber]
ATGATCCGCCGAGCGCAGATCCACTGGGTCGATCTGGACCCGGCGCAGGGTGGCGAAGCGGCCGAGCGTCGGCCGGCAGT
GGTGGTAAGCAACGACCGCGCCAACGCGACCGCCGAGAACCTGGGCAGAGGAGTGGTCACCGTCGTTCCTCTGATCGCCA
GCACCTCACACGTCTTTCCGTTCCAGGTACTCGTGGAACCCGACGAGTCGGGTCTGACCAGAGCGTCGAAAGCTCAGGCA
GAACAGGTACGTTCGGTGTCCGTGCGCCGCCTGGACGCCGAACCTGTCGGAAAACTCGGGGTGAGAACACTCGCAGCGCT
CGACGAAGCTCTACGGCTGCATCTCGATCTGCGCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7680.46 Da
• Isoelectric Point: 4.1694

>AT89154 WP_040270291.1 NZ_CP024890:c3809457-3809245 [Rhodococcus ruber]
MTVKFTISLPDEDVHVLDEYIRDHGLRGRSAGVRAAVRLLAAAGLERDYATAFAEVDTDDAWDVTTADTE

Nucleotide

• Length: 213 bp

>AT89154 NZ_CP024890:c3809457-3809245 [Rhodococcus ruber]
ATGACCGTCAAGTTCACGATCAGTCTCCCGGACGAGGATGTCCACGTCCTCGACGAGTACATCCGGGATCATGGGCTCCG
CGGCCGGTCGGCGGGCGTCCGAGCCGCTGTCCGCCTGCTCGCGGCAGCCGGACTCGAACGGGACTATGCCACGGCCTTTG
CCGAGGTCGACACGGACGACGCGTGGGACGTCACCACGGCCGACACCGAATGA

Structures

Toxin

• AlphaFold DB: A0A098BGQ9