Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2751969..2752587 Replicon chromosome
Accession NZ_CP024889
Organism Escherichia coli strain AR_0019

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM346_RS14600 Protein ID WP_001291435.1
Coordinates 2752369..2752587 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM346_RS14595 Protein ID WP_000344800.1
Coordinates 2751969..2752343 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM346_RS14585 2747058..2748251 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM346_RS14590 2748274..2751423 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM346_RS14595 2751969..2752343 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM346_RS14600 2752369..2752587 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM346_RS14605 2752759..2753310 + 552 WP_000102564.1 maltose O-acetyltransferase -
AM346_RS14610 2753426..2753896 + 471 WP_000136192.1 YlaC family protein -
AM346_RS14615 2754060..2755610 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM346_RS14620 2755652..2756005 - 354 WP_000878141.1 DUF1428 family protein -
AM346_RS14630 2756384..2756695 + 312 WP_000409911.1 MGMT family protein -
AM346_RS14635 2756726..2757298 - 573 WP_000779839.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T89146 WP_001291435.1 NZ_CP024889:2752369-2752587 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T89146 NZ_CP024889:2752369-2752587 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT89146 WP_000344800.1 NZ_CP024889:2751969-2752343 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT89146 NZ_CP024889:2751969-2752343 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References