Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 267687..268341 Replicon chromosome
Accession NZ_CP024889
Organism Escherichia coli strain AR_0019

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag AM346_RS01955 Protein ID WP_000244772.1
Coordinates 267934..268341 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AM346_RS01950 Protein ID WP_000354046.1
Coordinates 267687..267953 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM346_RS01925 262975..263718 + 744 WP_000951940.1 SDR family oxidoreductase -
AM346_RS01930 263775..265208 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
AM346_RS01935 265253..265564 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AM346_RS01940 265728..266387 + 660 WP_000250274.1 hemolysin III family protein -
AM346_RS01945 266464..267444 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AM346_RS01950 267687..267953 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AM346_RS01955 267934..268341 + 408 WP_000244772.1 protein YgfX Toxin
AM346_RS01960 268381..268902 - 522 WP_001055874.1 flavodoxin FldB -
AM346_RS01965 269014..269910 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AM346_RS01970 269935..270645 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM346_RS01975 270651..272384 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T89132 WP_000244772.1 NZ_CP024889:267934-268341 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T89132 NZ_CP024889:267934-268341 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT89132 WP_000354046.1 NZ_CP024889:267687-267953 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT89132 NZ_CP024889:267687-267953 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References