Detailed information of TA system TA089118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4281964..4282540
• Replicon: chromosome
• Accession: NZ_CP024886
• Organism: Escherichia coli strain AR_0017

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: AM344_RS22470
• Protein ID: WP_001297643.1
• Coordinates: 4281964..4282251 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: AM344_RS22475
• Protein ID: WP_021538262.1
• Coordinates: 4282238..4282540 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM344_RS22450	4278217..4279497	-	1281	WP_001037380.1	DUF445 domain-containing protein	-
AM344_RS22455	4279538..4280770	-	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
AM344_RS22460	4280916..4281011	-	96	Protein_4108	dienelactone hydrolase family protein	-
AM344_RS22465	4281068..4281765	+	698	Protein_4109	IS1 family transposase	-
AM344_RS22470	4281964..4282251	+	288	WP_001297643.1	BrnT family toxin	Toxin
AM344_RS22475	4282238..4282540	+	303	WP_021538262.1	BrnA antitoxin family protein	Antitoxin
AM344_RS22480	4282574..4283530	-	957	WP_001297640.1	GTPase	-
AM344_RS22485	4283541..4283744	-	204	WP_000467859.1	YbdD/YjiX family protein	-
AM344_RS22490	4283794..4285944	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T89118 WP_001297643.1 NZ_CP024886:4281964-4282251 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T89118 NZ_CP024886:4281964-4282251 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11531.00 Da
• Isoelectric Point: 9.4651

>AT89118 WP_021538262.1 NZ_CP024886:4282238-4282540 [Escherichia coli]
MSMVKHKRGTSSTLDVHHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT89118 NZ_CP024886:4282238-4282540 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCATCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9