Detailed information of TA system TA089117

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4245473..4246305
• Replicon: chromosome
• Accession: NZ_CP024886
• Organism: Escherichia coli strain AR_0017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: AM344_RS22240
• Protein ID: WP_000854754.1
• Coordinates: 4245931..4246305 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: AM344_RS22235
• Protein ID: WP_001315620.1
• Coordinates: 4245473..4245841 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM344_RS22200	4242310..4242765	+	456	WP_000581493.1	hypothetical protein	-
AM344_RS27480	4242884..4243095	-	212	Protein_4065	hypothetical protein	-
AM344_RS22215	4243176..4243994	+	819	WP_001513427.1	DUF945 domain-containing protein	-
AM344_RS22220	4244049..4244534	+	486	WP_000849588.1	antirestriction protein	-
AM344_RS22225	4244550..4245026	+	477	WP_001560709.1	RadC family protein	-
AM344_RS22230	4245089..4245310	+	222	WP_000692345.1	DUF987 domain-containing protein	-
AM344_RS22235	4245473..4245841	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM344_RS22240	4245931..4246305	+	375	WP_000854754.1	TA system toxin CbtA family protein	Toxin
AM344_RS22245	4246302..4246790	+	489	WP_100273043.1	hypothetical protein	-
AM344_RS22250	4246802..4246999	+	198	WP_000839281.1	DUF957 domain-containing protein	-
AM344_RS27485	4247084..4247236	+	153	WP_001280445.1	hypothetical protein	-
AM344_RS22275	4248273..4248923	+	651	WP_001037966.1	HNH endonuclease	-
AM344_RS22280	4248931..4249911	-	981	WP_001295601.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
AM344_RS22285	4249976..4251082	-	1107	WP_001315214.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14019.97 Da
• Isoelectric Point: 8.2905

>T89117 WP_000854754.1 NZ_CP024886:4245931-4246305 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEKR

Nucleotide

• Length: 375 bp

>T89117 NZ_CP024886:4245931-4246305 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT89117 WP_001315620.1 NZ_CP024886:4245473-4245841 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89117 NZ_CP024886:4245473-4245841 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: V0ULY5