Detailed information of TA system TA089115

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3899749..3900584
• Replicon: chromosome
• Accession: NZ_CP024886
• Organism: Escherichia coli strain AR_0017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VR61
• Locus tag: AM344_RS20225
• Protein ID: WP_000854747.1
• Coordinates: 3899749..3900126 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3P1Z1K5
• Locus tag: AM344_RS20230
• Protein ID: WP_021538665.1
• Coordinates: 3900216..3900584 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM344_RS20200	3895841..3897379	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
AM344_RS20210	3898116..3898970	-	855	WP_001588930.1	DUF4942 domain-containing protein	-
AM344_RS20215	3899055..3899252	-	198	WP_000839291.1	DUF957 domain-containing protein	-
AM344_RS20220	3899264..3899752	-	489	WP_000761667.1	hypothetical protein	-
AM344_RS20225	3899749..3900126	-	378	WP_000854747.1	TA system toxin CbtA family protein	Toxin
AM344_RS20230	3900216..3900584	-	369	WP_021538665.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM344_RS20235	3900659..3900880	-	222	WP_001825901.1	DUF987 domain-containing protein	-
AM344_RS20240	3900943..3901419	-	477	WP_001186747.1	RadC family protein	-
AM344_RS20245	3901435..3901920	-	486	WP_021538211.1	antirestriction protein	-
AM344_RS20250	3902012..3902830	-	819	WP_001234693.1	DUF945 domain-containing protein	-
AM344_RS20260	3902931..3903164	-	234	WP_021538212.1	DUF905 family protein	-
AM344_RS20265	3903170..3903847	-	678	WP_001097364.1	hypothetical protein	-
AM344_RS20270	3903966..3904850	-	885	WP_000010387.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3888352..3913331	24979

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14095.03 Da
• Isoelectric Point: 6.8524

>T89115 WP_000854747.1 NZ_CP024886:c3900126-3899749 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T89115 NZ_CP024886:c3900126-3899749 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACCCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATGACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13465.18 Da
• Isoelectric Point: 6.2015

>AT89115 WP_021538665.1 NZ_CP024886:c3900584-3900216 [Escherichia coli]
VSDTFSGTTHPDDNRGRPWWGLPCTVTPCFGARLVQEGNQLHYLADHAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89115 NZ_CP024886:c3900584-3900216 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGACGACAATCGCGGCCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCACGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGATACCCTCGGCAGCTG
TGGTTGCGTTTATCTGGCTGTTTATCCGACACCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2VFZ1

Antitoxin

• AlphaFold DB: A0A3P1Z1K5