Detailed information of TA system TA089108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1374137..1374615
• Replicon: chromosome
• Accession: NZ_CP024886
• Organism: Escherichia coli strain AR_0017

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: D8A5B3
• Locus tag: AM344_RS07650
• Protein ID: WP_000936799.1
• Coordinates: 1374328..1374615 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: D8A5B4
• Locus tag: AM344_RS07645
• Protein ID: WP_000100899.1
• Coordinates: 1374137..1374328 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM344_RS07595	1369205..1369561	-	357	WP_021538043.1	hypothetical protein	-
AM344_RS07600	1369539..1370000	-	462	WP_001209475.1	ead/Ea22-like family protein	-
AM344_RS07605	1369997..1370293	-	297	WP_024188212.1	DUF4406 domain-containing protein	-
AM344_RS07610	1370290..1370571	-	282	WP_072146789.1	DNA-binding protein	-
AM344_RS07615	1370604..1371320	-	717	WP_100272965.1	DUF1627 domain-containing protein	-
AM344_RS27145	1371354..1371896	-	543	WP_160799770.1	replication protein	-
AM344_RS27150	1371808..1372845	-	1038	WP_021538047.1	hypothetical protein	-
AM344_RS07630	1372917..1373342	-	426	WP_000693918.1	Rha family transcriptional regulator	-
AM344_RS07635	1373326..1373598	-	273	WP_000887447.1	helix-turn-helix domain-containing protein	-
AM344_RS07640	1373708..1374109	+	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
AM344_RS07645	1374137..1374328	+	192	WP_000100899.1	hypothetical protein	Antitoxin
AM344_RS07650	1374328..1374615	+	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM344_RS07660	1374889..1375041	+	153	WP_021538048.1	DUF1391 family protein	-
AM344_RS07665	1375053..1375391	+	339	WP_000394541.1	hypothetical protein	-
AM344_RS07670	1375380..1375646	-	267	WP_012601410.1	hypothetical protein	-
AM344_RS07680	1376150..1376338	+	189	WP_000449172.1	cell division inhibitor	-
AM344_RS07685	1376335..1376523	+	189	WP_000199480.1	DUF1482 family protein	-
AM344_RS07690	1376619..1379093	+	2475	WP_021538049.1	exonuclease	-
AM344_RS07695	1379152..1379355	+	204	WP_000096346.1	DUF4224 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtS / ybtX / ybtQ / ybtP	1330744..1389313	58569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10929.85 Da
• Isoelectric Point: 10.1360

>T89108 WP_000936799.1 NZ_CP024886:1374328-1374615 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENIPAARRLKIRIETSVLALSEHPYLYPPSDRVSGLREIVVHPNYIVLYRVATSSIE
IANIVHARRQFPFPI

Nucleotide

• Length: 288 bp

>T89108 NZ_CP024886:1374328-1374615 [Escherichia coli]
ATGTTACCCATTTTGTGGCTACCATCTGCTCGCGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAATATTCC
TGCAGCACGCAGACTAAAAATACGGATTGAAACGTCTGTTTTAGCCCTCTCTGAGCATCCATATCTATATCCGCCAAGTG
ATCGAGTATCCGGTTTGCGGGAAATTGTGGTTCACCCTAATTATATCGTTTTGTACCGAGTAGCAACTTCAAGCATTGAA
ATTGCAAATATTGTGCATGCCCGCCGACAATTTCCCTTCCCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7035.80 Da
• Isoelectric Point: 4.0270

>AT89108 WP_000100899.1 NZ_CP024886:1374137-1374328 [Escherichia coli]
MSTALSPIVSEFETTEQENSYNEWLRAKVASSLADPRPSIPHDEVMAEMENLIAQIAVTNRSE

Nucleotide

• Length: 192 bp

>AT89108 NZ_CP024886:1374137-1374328 [Escherichia coli]
ATGAGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTACGCGC
TAAAGTGGCGTCAAGCCTTGCAGACCCTCGTCCCTCAATTCCACATGATGAGGTAATGGCTGAAATGGAGAATCTTATTG
CTCAAATTGCTGTAACTAACAGGAGCGAGTAA

Structures

Toxin

• AlphaFold DB: D8A5B3

Antitoxin

• AlphaFold DB: A0A161RC07