Detailed information of TA system TA089074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1435501..1436522
• Replicon: chromosome
• Accession: NZ_CP024885
• Organism: Klebsiella aerogenes strain AR_0009

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A0G9UXE3
• Locus tag: AM336_RS07355
• Protein ID: WP_032706547.1
• Coordinates: 1435501..1436076 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0G9R618
• Locus tag: AM336_RS07360
• Protein ID: WP_015369110.1
• Coordinates: 1436076..1436522 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM336_RS07340	1432786..1433823	+	1038	WP_032706544.1	virulence RhuM family protein	-
AM336_RS07345	1434000..1435196	-	1197	WP_015369112.1	hypothetical protein	-
AM336_RS07355	1435501..1436076	-	576	WP_032706547.1	PIN domain-containing protein	Toxin
AM336_RS07360	1436076..1436522	-	447	WP_015369110.1	helix-turn-helix domain-containing protein	Antitoxin
AM336_RS07365	1436733..1437740	+	1008	WP_015369109.1	zinc-binding alcohol dehydrogenase family protein	-
AM336_RS07370	1437741..1438043	+	303	WP_015369108.1	antibiotic biosynthesis monooxygenase	-
AM336_RS07375	1438201..1439250	+	1050	WP_015369107.1	NAD(P)-dependent alcohol dehydrogenase	-
AM336_RS07380	1439247..1440200	-	954	WP_032706577.1	helix-turn-helix domain-containing protein	-
AM336_RS07385	1440222..1441001	-	780	WP_015369105.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21688.29 Da
• Isoelectric Point: 6.2900

>T89074 WP_032706547.1 NZ_CP024885:c1436076-1435501 [Klebsiella aerogenes]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Nucleotide

• Length: 576 bp

>T89074 NZ_CP024885:c1436076-1435501 [Klebsiella aerogenes]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCTTGTGTATTATATCCAGCACGGCTCAGGGATTTATTAATGCACTT
AGGGATCGCTGGACTTTATCAACCAAAATGGAGCCGCTTCATTCACGATGAATGGTGCAGGAATCTTCTTATAAATCGGC
CTGATATAGCACCTGAAGCGCTGCGTCGTACCGTTGATCTCATGAATGCAGCTTTACCGGATGCTAATGTAACGGGTTTT
GCAGGATTAATTAACGGACTAGTTTTACCCGACCCTGACGATCGGCATGTCCTTGCTGCGGCAATCAGAGCGAAGGCTGA
AATTATCGTTACGTTAAATCATAAAGACTTTCCAGCAGAATCATTGCTTCCATTTGAAATCGAAACACTTCATCCTGATG
TATTCATATCGGACCTGTTTGACCTTAATCATGCTCTGGCGTTGGAGGCGGTGAGAAGGCAGCGGCAGTCTCTCAAGCAT
CCTCCAATGACCGTCGATGAGTTCCTGGAGATGCTGCTAAAGCAGGGCTTGCCGATGACGGTAAAAGAGTTAGCCAATTA
CCAGTACGCCATATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16699.26 Da
• Isoelectric Point: 6.2257

>AT89074 WP_015369110.1 NZ_CP024885:c1436522-1436076 [Klebsiella aerogenes]
MTKLNLPDPLESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Nucleotide

• Length: 447 bp

>AT89074 NZ_CP024885:c1436522-1436076 [Klebsiella aerogenes]
ATGACTAAACTGAACCTTCCGGACCCGCTGGAGAGTGAACTTGCCCAGCGCGGGCAGCGTGAGCTGGCGGCATACCTTTC
TACTCATCTTGAAACGCAGCGTATTGCGATTGTGGGTGAAGATGACAAAACTCATACCATTGAGTTGCCAACCAGCGCTC
TGACTATGCTGATGGAGATCCTTGGGGAATTGGCTTGTGGCAACGCGGTGCAGATTGTTCCTGTTCATGCTGAACTCACC
ACCCAGGAAGCAGCGAATATACTCAATGTTTCTCGTCCGCATATGGTTAAACTGCTGGAAGCGAGGAAGCTTCCATTTCA
TAAAACTGGACGCCATCGCCGGGTTCTGTTTGCCGATCTGATGGAGTATAAAAAACGTAGGGAACAGGAAAGCCTGGACG
CGATGCAAGCGCTGGCGGATCAGGCGCAGGACTTAGGGATGTATTAA

Structures

Toxin

• AlphaFold DB: A0A0G9UXE3

Antitoxin

• AlphaFold DB: A0A0G9R618