Detailed information of TA system TA089070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3729471..3730210
• Replicon: chromosome
• Accession: NZ_CP024883
• Organism: Klebsiella aerogenes strain AR_0007

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: AM334_RS18405
• Protein ID: WP_032705770.1
• Coordinates: 3729725..3730210 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: AM334_RS18400
• Protein ID: WP_015705366.1
• Coordinates: 3729471..3729737 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM334_RS18375	3724806..3725393	+	588	WP_100279812.1	hypothetical protein	-
AM334_RS18380	3725390..3726709	+	1320	WP_100279813.1	two-component sensor histidine kinase	-
AM334_RS18385	3726709..3727326	+	618	WP_032711621.1	response regulator transcription factor	-
AM334_RS18390	3727422..3727919	+	498	WP_015705368.1	heme-binding protein	-
AM334_RS18395	3727963..3729198	-	1236	WP_100279814.1	MFS transporter	-
AM334_RS18400	3729471..3729737	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
AM334_RS18405	3729725..3730210	+	486	WP_032705770.1	GNAT family N-acetyltransferase	Toxin
AM334_RS18410	3730282..3731898	+	1617	WP_047038622.1	FAD-NAD(P)-binding protein	-
AM334_RS18415	3732017..3733117	+	1101	WP_015705363.1	NADH:flavin oxidoreductase/NADH oxidase	-
AM334_RS18420	3733174..3733332	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
AM334_RS18425	3733593..3734663	+	1071	WP_015705361.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17573.23 Da
• Isoelectric Point: 8.8793

>T89070 WP_032705770.1 NZ_CP024883:3729725-3730210 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHSEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T89070 NZ_CP024883:3729725-3730210 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACTCGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTTTCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTGTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACTCTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT89070 WP_015705366.1 NZ_CP024883:3729471-3729737 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT89070 NZ_CP024883:3729471-3729737 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCGCGGACCGACTTTATCCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGTCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9