Detailed information of TA system TA089057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 102418..102944
• Replicon: plasmid unitig_1_pilon
• Accession: NZ_CP024882
• Organism: Citrobacter freundii strain AR_0022

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AM349_RS24785
• Protein ID: WP_000323025.1
• Coordinates: 102657..102944 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AM349_RS24780
• Protein ID: WP_000534858.1
• Coordinates: 102418..102657 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM349_RS24745	97574..97924	+	351	Protein_99	DUF1281 domain-containing protein	-
AM349_RS24750	97923..98183	+	261	WP_100280323.1	hypothetical protein	-
-	98232..98356	+	125	NuclAT_0	-	-
-	98232..98356	+	125	NuclAT_0	-	-
-	98232..98356	+	125	NuclAT_0	-	-
-	98232..98356	+	125	NuclAT_0	-	-
AM349_RS24760	98477..99817	+	1341	WP_058671058.1	ISNCY family transposase	-
AM349_RS24765	100460..101032	+	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
AM349_RS24770	101232..102155	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
AM349_RS24780	102418..102657	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AM349_RS24785	102657..102944	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AM349_RS24790	103016..103174	+	159	WP_079897042.1	type I toxin-antitoxin system Hok family toxin	-
AM349_RS25010	103217..103396	+	180	WP_131403754.1	hypothetical protein	-
AM349_RS24805	103955..104248	+	294	WP_061548396.1	hypothetical protein	-
AM349_RS24810	104260..105081	+	822	WP_061548395.1	DUF945 domain-containing protein	-
AM349_RS24815	105111..105644	-	534	WP_061548394.1	lytic transglycosylase domain-containing protein	-
AM349_RS24820	106299..106691	+	393	WP_100280319.1	relaxosome protein TraM	-
AM349_RS24825	106723..107364	-	642	WP_061548344.1	Arc family DNA-binding protein	-
AM349_RS24830	107563..107916	+	354	WP_061548345.1	conjugal transfer protein TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..115736	115736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T89057 WP_000323025.1 NZ_CP024882:102657-102944 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T89057 NZ_CP024882:102657-102944 [Citrobacter freundii]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT89057 WP_000534858.1 NZ_CP024882:102418-102657 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT89057 NZ_CP024882:102418-102657 [Citrobacter freundii]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8