Detailed information of TA system TA089056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4662988..4663608
• Replicon: chromosome
• Accession: NZ_CP024881
• Organism: Citrobacter freundii strain AR_0022

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: AM349_RS23000
• Protein ID: WP_002892050.1
• Coordinates: 4662988..4663206 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: AM349_RS23005
• Protein ID: WP_032948706.1
• Coordinates: 4663234..4663608 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM349_RS22965	4658346..4658975	+	630	WP_032948704.1	membrane protein	-
AM349_RS22970	4659040..4659393	+	354	WP_032948705.1	DUF1428 family protein	-
AM349_RS22975	4659445..4660998	-	1554	WP_003835927.1	EAL domain-containing protein	-
AM349_RS22980	4661187..4661447	+	261	WP_003021719.1	type B 50S ribosomal protein L31	-
AM349_RS22985	4661449..4661589	+	141	WP_005125190.1	type B 50S ribosomal protein L36	-
AM349_RS22990	4661668..4662138	-	471	WP_003021724.1	YlaC family protein	-
AM349_RS22995	4662255..4662806	-	552	WP_003021728.1	maltose O-acetyltransferase	-
AM349_RS23000	4662988..4663206	-	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
AM349_RS23005	4663234..4663608	-	375	WP_032948706.1	Hha toxicity modulator TomB	Antitoxin
AM349_RS23010	4664096..4667245	-	3150	WP_048216755.1	efflux RND transporter permease AcrB	-
AM349_RS23015	4667268..4668461	-	1194	WP_003835922.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T89056 WP_002892050.1 NZ_CP024881:c4663206-4662988 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T89056 NZ_CP024881:c4663206-4662988 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14408.18 Da
• Isoelectric Point: 5.5154

>AT89056 WP_032948706.1 NZ_CP024881:c4663608-4663234 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFHCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT89056 NZ_CP024881:c4663608-4663234 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCACTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2