Detailed information of TA system TA089055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3729088..3729754
• Replicon: chromosome
• Accession: NZ_CP024881
• Organism: Citrobacter freundii strain AR_0022

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A8B5QBX6
• Locus tag: AM349_RS18535
• Protein ID: WP_032948292.1
• Coordinates: 3729088..3729447 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: AM349_RS18540
• Protein ID: WP_003031625.1
• Coordinates: 3729437..3729754 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM349_RS18525	3726488..3728119	+	1632	WP_003031618.1	Na/Pi cotransporter family protein	-
AM349_RS18530	3728185..3728874	-	690	WP_003841421.1	dipeptidase PepE	-
AM349_RS18535	3729088..3729447	+	360	WP_032948292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM349_RS18540	3729437..3729754	+	318	WP_003031625.1	helix-turn-helix domain-containing protein	Antitoxin
AM349_RS18545	3729764..3730042	-	279	WP_032948293.1	putative addiction module antidote protein	-
AM349_RS18550	3730446..3730607	-	162	WP_003841416.1	phage protein	-
AM349_RS18555	3730749..3731105	+	357	WP_032948294.1	hypothetical protein	-
AM349_RS18560	3731157..3731969	-	813	WP_032948295.1	shikimate 5-dehydrogenase	-
AM349_RS18565	3731971..3733194	-	1224	WP_032948296.1	L-sorbose 1-phosphate reductase	-
AM349_RS18570	3733262..3734086	-	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13461.47 Da
• Isoelectric Point: 10.2555

>T89055 WP_032948292.1 NZ_CP024881:3729088-3729447 [Citrobacter freundii]
MTKPLYWVGHARKDLQGMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNTWRAVYTIQLKNAVYVL
HVFQKKSVAGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T89055 NZ_CP024881:3729088-3729447 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCATGCCAGGAAAGATCTACAGGGAATGCCAGAACATGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGCGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACACATGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTGCCGGGAAGGCAACGCCCAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11522.46 Da
• Isoelectric Point: 9.0005

>AT89055 WP_003031625.1 NZ_CP024881:3729437-3729754 [Citrobacter freundii]
MSHEVEASSGNVYADLGVYDAEQMQLKAQLAMVIGEILQRKGLTQQQSAQLLGMTQPKLSNLLRGQFRGISEVKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT89055 NZ_CP024881:3729437-3729754 [Citrobacter freundii]
ATGTCACATGAAGTTGAAGCCAGTAGCGGAAATGTTTACGCCGATTTAGGCGTGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGTCCGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGCTACGTGGTCAGTTTCGTGGTATCAGTGAAGTAAAGATGCTCGAATGCCTGGTG
AGACTGGGTCGTGATGTAGAGATTGTTGTTGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A8B5QBX6