Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3576604..3577220 Replicon chromosome
Accession NZ_CP024881
Organism Citrobacter freundii strain AR_0022

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag AM349_RS17805 Protein ID WP_003028682.1
Coordinates 3576846..3577220 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag AM349_RS17800 Protein ID WP_043018956.1
Coordinates 3576604..3576846 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM349_RS17770 3572454..3573053 + 600 WP_016151258.1 glucose-1-phosphatase -
AM349_RS17775 3573047..3573919 + 873 WP_003028669.1 virulence factor BrkB family protein -
AM349_RS17780 3573916..3574353 + 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
AM349_RS17785 3574398..3575339 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
AM349_RS17795 3575490..3576398 - 909 WP_032950680.1 alpha/beta hydrolase -
AM349_RS17800 3576604..3576846 + 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
AM349_RS17805 3576846..3577220 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
AM349_RS17810 3577257..3578186 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
AM349_RS17815 3578183..3578818 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
AM349_RS17820 3578815..3579717 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T89054 WP_003028682.1 NZ_CP024881:3576846-3577220 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T89054 NZ_CP024881:3576846-3577220 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT89054 WP_043018956.1 NZ_CP024881:3576604-3576846 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT89054 NZ_CP024881:3576604-3576846 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References