Detailed information of TA system TA089053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3142168..3142754
• Replicon: chromosome
• Accession: NZ_CP024881
• Organism: Citrobacter freundii strain AR_0022

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0J1QS94
• Locus tag: AM349_RS15670
• Protein ID: WP_032937237.1
• Coordinates: 3142168..3142536 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0J1N092
• Locus tag: AM349_RS15675
• Protein ID: WP_032937236.1
• Coordinates: 3142533..3142754 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM349_RS15650	3137705..3138775	-	1071	WP_032950429.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
AM349_RS15655	3138778..3139623	-	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
AM349_RS15660	3139620..3140507	-	888	WP_032950430.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
AM349_RS15665	3140608..3141924	-	1317	WP_003837839.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
AM349_RS15670	3142168..3142536	-	369	WP_032937237.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM349_RS15675	3142533..3142754	-	222	WP_032937236.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AM349_RS15680	3142876..3143157	-	282	WP_003023442.1	hypothetical protein	-
AM349_RS15685	3143260..3143973	-	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
AM349_RS15690	3143991..3144758	-	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
AM349_RS15695	3144755..3146032	-	1278	WP_032950432.1	branched chain amino acid ABC transporter permease LivM	-
AM349_RS15700	3146029..3146955	-	927	WP_003023435.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3136965..3146032	9067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.91 Da
• Isoelectric Point: 6.9891

>T89053 WP_032937237.1 NZ_CP024881:c3142536-3142168 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Nucleotide

• Length: 369 bp

>T89053 NZ_CP024881:c3142536-3142168 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8306.41 Da
• Isoelectric Point: 4.7906

>AT89053 WP_032937236.1 NZ_CP024881:c3142754-3142533 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT89053 NZ_CP024881:c3142754-3142533 [Citrobacter freundii]
ATGCGCACTATGAACTATAGTGAAGCTCGACAAAACCTGGCTGAAGTGCTGGAGAGCGCTGTCACTGGTGTGCCTGTTAC
CATTACGCGCCGTGGTCATAAACCTGCGGTAATCATCAGCGCGGAAGAGTTTGAACGCTATCAGGCCGCCAGACTTGATG
ATGAATTTGCCGACATAATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0J1QS94

Antitoxin

• AlphaFold DB: A0A0J1N092