Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2497822..2498476 Replicon chromosome
Accession NZ_CP024881
Organism Citrobacter freundii strain AR_0022

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag AM349_RS12290 Protein ID WP_003026936.1
Coordinates 2497822..2498229 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag AM349_RS12295 Protein ID WP_003026938.1
Coordinates 2498210..2498476 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM349_RS12270 2493770..2495503 - 1734 WP_032950023.1 single-stranded-DNA-specific exonuclease RecJ -
AM349_RS12275 2495509..2496222 - 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM349_RS12280 2496246..2497142 - 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
AM349_RS12285 2497256..2497777 + 522 WP_003026933.1 flavodoxin FldB -
AM349_RS12290 2497822..2498229 - 408 WP_003026936.1 protein YgfX Toxin
AM349_RS12295 2498210..2498476 - 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
AM349_RS12305 2498733..2499713 + 981 WP_003026944.1 tRNA-modifying protein YgfZ -
AM349_RS12310 2499807..2500466 - 660 WP_003026947.1 hemolysin III family protein -
AM349_RS12315 2500629..2500940 - 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
AM349_RS12320 2500993..2501721 + 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
AM349_RS12325 2501936..2502445 + 510 WP_044713501.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T89052 WP_003026936.1 NZ_CP024881:c2498229-2497822 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T89052 NZ_CP024881:c2498229-2497822 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGCCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT89052 WP_003026938.1 NZ_CP024881:c2498476-2498210 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT89052 NZ_CP024881:c2498476-2498210 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References