Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 2447429..2448129 Replicon chromosome
Accession NZ_CP024881
Organism Citrobacter freundii strain AR_0022

Toxin (Protein)


Gene name ypjF Uniprot ID A0A0H3GY64
Locus tag AM349_RS12020 Protein ID WP_014226745.1
Coordinates 2447429..2447752 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7G2IQI9
Locus tag AM349_RS12025 Protein ID WP_032949996.1
Coordinates 2447773..2448129 (-) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM349_RS12010 2443372..2445834 - 2463 WP_032949991.1 poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA -
AM349_RS12015 2446651..2447187 + 537 WP_032949993.1 GNAT family N-acetyltransferase -
AM349_RS12020 2447429..2447752 - 324 WP_014226745.1 TA system toxin CbtA family protein Toxin
AM349_RS12025 2447773..2448129 - 357 WP_032949996.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AM349_RS12030 2448155..2448376 - 222 WP_014226747.1 DUF987 domain-containing protein -
AM349_RS12035 2448373..2448915 - 543 WP_032949998.1 DNA repair protein RadC -
AM349_RS12040 2448928..2449371 - 444 WP_014226749.1 antirestriction protein -
AM349_RS12045 2449402..2450223 - 822 WP_014226750.1 DUF945 domain-containing protein -
AM349_RS12050 2450322..2450552 - 231 WP_014226751.1 DUF905 domain-containing protein -
AM349_RS12055 2450624..2451073 - 450 WP_032949999.1 hypothetical protein -
AM349_RS12060 2451070..2451522 - 453 WP_000737933.1 hypothetical protein -
AM349_RS12065 2451559..2452128 - 570 WP_032950001.1 hypothetical protein -
AM349_RS12070 2452128..2452832 - 705 WP_014226755.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12466.52 Da        Isoelectric Point: 7.1331

>T89051 WP_014226745.1 NZ_CP024881:c2447752-2447429 [Citrobacter freundii]
MKFLPATNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Download         Length: 324 bp

>T89051 NZ_CP024881:c2447752-2447429 [Citrobacter freundii]
ATGAAATTTCTACCTGCAACAAATATGCGGGCGGCGAAGCCATGCCTGTCGCCCGTGACTATCTGGCAGATGCTACTTAG
TCGTCTGCTGGAACAGCATTATGGCCTGACACTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGATTATTGATATCATGAGGGCCCGTCGCGATCTGGGTTTAATGAATCGTAA
TTAG

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13119.92 Da        Isoelectric Point: 6.9376

>AT89051 WP_032949996.1 NZ_CP024881:c2448129-2447773 [Citrobacter freundii]
MNNRSESGAIPENPACQQWGLKRAMTPCFGARLVQEGARLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELILTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYITIYPTQR

Download         Length: 357 bp

>AT89051 NZ_CP024881:c2448129-2447773 [Citrobacter freundii]
ATGAACAATCGCTCAGAATCCGGTGCCATACCGGAGAATCCCGCCTGCCAGCAATGGGGGCTGAAACGTGCTATGACCCC
CTGTTTTGGAGCACGTCTGGTGCAGGAAGGCGCCCGCCTGCATTTTCTCGCGGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTCGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGCTGATACTCACCAGCGGAGAA
CTCTCCCCCCGCCATCAGCACTGTGTCACCCTCTATCACAACGGACTCACCTGTGAAGCCGATACGCTTGGTAGTTGTGG
CTACGTATACATCACCATTTATCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GY64

Antitoxin

Source ID Structure
AlphaFold DB A0A7G2IQI9

References