Detailed information of TA system TA089051

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 2447429..2448129
• Replicon: chromosome
• Accession: NZ_CP024881
• Organism: Citrobacter freundii strain AR_0022

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0H3GY64
• Locus tag: AM349_RS12020
• Protein ID: WP_014226745.1
• Coordinates: 2447429..2447752 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7G2IQI9
• Locus tag: AM349_RS12025
• Protein ID: WP_032949996.1
• Coordinates: 2447773..2448129 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM349_RS12010	2443372..2445834	-	2463	WP_032949991.1	poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA	-
AM349_RS12015	2446651..2447187	+	537	WP_032949993.1	GNAT family N-acetyltransferase	-
AM349_RS12020	2447429..2447752	-	324	WP_014226745.1	TA system toxin CbtA family protein	Toxin
AM349_RS12025	2447773..2448129	-	357	WP_032949996.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM349_RS12030	2448155..2448376	-	222	WP_014226747.1	DUF987 domain-containing protein	-
AM349_RS12035	2448373..2448915	-	543	WP_032949998.1	DNA repair protein RadC	-
AM349_RS12040	2448928..2449371	-	444	WP_014226749.1	antirestriction protein	-
AM349_RS12045	2449402..2450223	-	822	WP_014226750.1	DUF945 domain-containing protein	-
AM349_RS12050	2450322..2450552	-	231	WP_014226751.1	DUF905 domain-containing protein	-
AM349_RS12055	2450624..2451073	-	450	WP_032949999.1	hypothetical protein	-
AM349_RS12060	2451070..2451522	-	453	WP_000737933.1	hypothetical protein	-
AM349_RS12065	2451559..2452128	-	570	WP_032950001.1	hypothetical protein	-
AM349_RS12070	2452128..2452832	-	705	WP_014226755.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12466.52 Da
• Isoelectric Point: 7.1331

>T89051 WP_014226745.1 NZ_CP024881:c2447752-2447429 [Citrobacter freundii]
MKFLPATNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Nucleotide

• Length: 324 bp

>T89051 NZ_CP024881:c2447752-2447429 [Citrobacter freundii]
ATGAAATTTCTACCTGCAACAAATATGCGGGCGGCGAAGCCATGCCTGTCGCCCGTGACTATCTGGCAGATGCTACTTAG
TCGTCTGCTGGAACAGCATTATGGCCTGACACTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGATTATTGATATCATGAGGGCCCGTCGCGATCTGGGTTTAATGAATCGTAA
TTAG

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13119.92 Da
• Isoelectric Point: 6.9376

>AT89051 WP_032949996.1 NZ_CP024881:c2448129-2447773 [Citrobacter freundii]
MNNRSESGAIPENPACQQWGLKRAMTPCFGARLVQEGARLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELILTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYITIYPTQR

Nucleotide

• Length: 357 bp

>AT89051 NZ_CP024881:c2448129-2447773 [Citrobacter freundii]
ATGAACAATCGCTCAGAATCCGGTGCCATACCGGAGAATCCCGCCTGCCAGCAATGGGGGCTGAAACGTGCTATGACCCC
CTGTTTTGGAGCACGTCTGGTGCAGGAAGGCGCCCGCCTGCATTTTCTCGCGGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTCGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGCTGATACTCACCAGCGGAGAA
CTCTCCCCCCGCCATCAGCACTGTGTCACCCTCTATCACAACGGACTCACCTGTGAAGCCGATACGCTTGGTAGTTGTGG
CTACGTATACATCACCATTTATCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GY64

Antitoxin

• AlphaFold DB: A0A7G2IQI9