Detailed information of TA system TA089042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 840812..841451
• Replicon: chromosome
• Accession: NZ_CP024881
• Organism: Citrobacter freundii strain AR_0022

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: AM349_RS04205
• Protein ID: WP_003020221.1
• Coordinates: 840812..840988 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM349_RS04210
• Protein ID: WP_123924900.1
• Coordinates: 841035..841451 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM349_RS04185	837590..837820	-	231	WP_003020233.1	DUF2554 family protein	-
AM349_RS04190	838010..838135	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
AM349_RS04195	838135..839145	-	1011	WP_100280127.1	cytochrome d ubiquinol oxidase subunit II	-
AM349_RS04200	839145..840548	-	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
AM349_RS04205	840812..840988	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM349_RS04210	841035..841451	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM349_RS04215	841544..842953	+	1410	WP_016150280.1	PLP-dependent aminotransferase family protein	-
AM349_RS04220	843282..844427	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
AM349_RS04225	844444..845460	+	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
AM349_RS04230	845461..846405	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T89042 WP_003020221.1 NZ_CP024881:840812-840988 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T89042 NZ_CP024881:840812-840988 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT89042 WP_123924900.1 NZ_CP024881:841035-841451 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT89042 NZ_CP024881:841035-841451 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACGAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36