Detailed information of TA system TA089029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2717653..2718392
• Replicon: chromosome
• Accession: NZ_CP024880
• Organism: Klebsiella aerogenes strain AR_0018

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: AM345_RS13380
• Protein ID: WP_100278924.1
• Coordinates: 2717653..2718138 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: AM345_RS13385
• Protein ID: WP_015705366.1
• Coordinates: 2718126..2718392 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM345_RS13360	2713200..2714270	-	1071	WP_045365882.1	mannonate dehydratase	-
AM345_RS13365	2714531..2714689	+	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
AM345_RS13370	2714746..2715846	-	1101	WP_015366663.1	NADH:flavin oxidoreductase/NADH oxidase	-
AM345_RS13375	2715965..2717581	-	1617	WP_048230873.1	FAD-NAD(P)-binding protein	-
AM345_RS13380	2717653..2718138	-	486	WP_100278924.1	GNAT family N-acetyltransferase	Toxin
AM345_RS13385	2718126..2718392	-	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
AM345_RS13390	2718665..2719900	+	1236	WP_020078885.1	MFS transporter	-
AM345_RS13395	2719944..2720441	-	498	WP_047076455.1	heme-binding protein	-
AM345_RS13400	2720537..2721154	-	618	WP_032711621.1	response regulator transcription factor	-
AM345_RS13405	2721154..2722473	-	1320	WP_048230874.1	two-component sensor histidine kinase	-
AM345_RS13410	2722470..2723057	-	588	WP_048230875.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (25-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17561.24 Da
• Isoelectric Point: 8.8793

>T89029 WP_100278924.1 NZ_CP024880:c2718138-2717653 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGMKNQAQGAARTFVVCKEDSHQVVGFYSLATGSVNHAEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T89029 NZ_CP024880:c2718138-2717653 [Klebsiella aerogenes]
ATGGGAAAAATAAGTGCGCCTGCACCGCTATCTTCACACCATCAGATTGCGGAGTTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTAAAACAACGAGGAATGAAAAACCAGGCTCAGGGTGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGATAGCC
ATCAGGTGGTGGGATTTTATTCGTTAGCAACCGGCAGCGTTAATCACGCTGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTTTCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAGCGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT89029 WP_015705366.1 NZ_CP024880:c2718392-2718126 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT89029 NZ_CP024880:c2718392-2718126 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCGCGGACCGACTTTATCCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGTCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9