Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1445781..1446400 Replicon chromosome
Accession NZ_CP024880
Organism Klebsiella aerogenes strain AR_0018

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag AM345_RS07090 Protein ID WP_015367918.1
Coordinates 1445781..1445999 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag AM345_RS07095 Protein ID WP_015367917.1
Coordinates 1446026..1446400 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM345_RS07060 1441854..1442114 + 261 WP_015367923.1 type B 50S ribosomal protein L31 -
AM345_RS07065 1442117..1442257 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM345_RS07070 1442254..1442964 - 711 WP_015704607.1 GNAT family N-acetyltransferase -
AM345_RS07075 1443067..1444506 + 1440 WP_047046909.1 PLP-dependent aminotransferase family protein -
AM345_RS07080 1444496..1444960 - 465 WP_047046911.1 YlaC family protein -
AM345_RS07085 1445086..1445649 - 564 WP_015704609.1 maltose O-acetyltransferase -
AM345_RS07090 1445781..1445999 - 219 WP_015367918.1 hemolysin expression modulator Hha Toxin
AM345_RS07095 1446026..1446400 - 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
AM345_RS07100 1446873..1450019 - 3147 WP_015367916.1 multidrug efflux RND transporter permease subunit -
AM345_RS07105 1450042..1451241 - 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1437116..1446400 9284


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T89028 WP_015367918.1 NZ_CP024880:c1445999-1445781 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T89028 NZ_CP024880:c1445999-1445781 [Klebsiella aerogenes]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATATGAACTTTCCGACAATGAATTGGCGGTATTTTACTCAGCAGCCGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCAACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT89028 WP_015367917.1 NZ_CP024880:c1446400-1446026 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT89028 NZ_CP024880:c1446400-1446026 [Klebsiella aerogenes]
ATGGACGAGTACTCGCCAAAAAGACATGATATCGCGCAGCTAAGATTTCTCTGCGAAACGTTGTATCATGACTGCCTTGC
AAACCTGGAAGAAAGCAACCGCGGCTGGGTTAACGATCCCACCTCGGCGGTGAATCTTCAATTAAATGAATTGATCGAGC
ACATAGCAACTTACGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATTTCTCAGCTTGACGAATACCTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAGCGATCTCCAAAAATGGCGCAAATCCGGCAATCGGTT
ATTCCGATGCTTTGTGAACGCCAGCCGGGAGAACCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References