Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 90184..90709 Replicon plasmid pNH25.2
Accession NZ_CP024876
Organism Klebsiella pneumoniae strain NH25

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag CU111_RS28775 Protein ID WP_013023785.1
Coordinates 90184..90489 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag CU111_RS28780 Protein ID WP_001568025.1
Coordinates 90491..90709 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU111_RS28745 85903..86529 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
CU111_RS28750 86649..86828 + 180 WP_006788217.1 hypothetical protein -
CU111_RS28755 87260..88054 - 795 WP_004197635.1 site-specific integrase -
CU111_RS28760 88252..89268 - 1017 WP_017899884.1 hypothetical protein -
CU111_RS28765 89279..89593 - 315 WP_053389906.1 hypothetical protein -
CU111_RS28770 89620..90015 - 396 WP_017899885.1 hypothetical protein -
CU111_RS28775 90184..90489 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
CU111_RS28780 90491..90709 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CU111_RS28800 91527..91817 - 291 WP_013023783.1 hypothetical protein -
CU111_RS28805 91814..92941 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
CU111_RS28810 92975..94567 - 1593 WP_015344964.1 hypothetical protein -
CU111_RS28815 94774..95553 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) / blaCTX-M-15 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / mph(A) - 1..129299 129299


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T89027 WP_013023785.1 NZ_CP024876:c90489-90184 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T89027 NZ_CP024876:c90489-90184 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT89027 WP_001568025.1 NZ_CP024876:c90709-90491 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT89027 NZ_CP024876:c90709-90491 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References