Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 82417..83069 Replicon plasmid pNH25.1
Accession NZ_CP024875
Organism Klebsiella pneumoniae strain NH25

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag CU111_RS27335 Protein ID WP_017901321.1
Coordinates 82417..82842 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag CU111_RS27340 Protein ID WP_001261275.1
Coordinates 82839..83069 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU111_RS27300 78126..78242 + 117 Protein_82 transposase -
CU111_RS27305 78367..78537 - 171 Protein_83 LysR family transcriptional regulator -
CU111_RS27310 78662..78775 + 114 WP_077252861.1 IS3 family transposase -
CU111_RS27315 78867..80438 - 1572 WP_049257520.1 sensor domain-containing diguanylate cyclase -
CU111_RS27325 80836..81396 - 561 Protein_86 transposase -
CU111_RS27330 81431..82399 - 969 WP_077254762.1 IS5 family transposase -
CU111_RS27335 82417..82842 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
CU111_RS27340 82839..83069 - 231 WP_001261275.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CU111_RS27350 83322..85898 - 2577 WP_017901322.1 EstP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / catA1 / dfrA5 / qacE - 1..227692 227692
- flank IS/Tn - - 81431..82399 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T89026 WP_017901321.1 NZ_CP024875:c82842-82417 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T89026 NZ_CP024875:c82842-82417 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT89026 WP_001261275.1 NZ_CP024875:c83069-82839 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT89026 NZ_CP024875:c83069-82839 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References