Detailed information of TA system TA089024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 12642..13552
• Replicon: plasmid pNH25.1
• Accession: NZ_CP024875
• Organism: Klebsiella pneumoniae strain NH25

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: CU111_RS26925
• Protein ID: WP_004144067.1
• Coordinates: 13082..13552 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6A8PHD0
• Locus tag: CU111_RS26920
• Protein ID: WP_023287184.1
• Coordinates: 12642..13085 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU111_RS26900	8058..8807	+	750	Protein_11	IS5 family transposase	-
CU111_RS26905	8836..9804	+	969	WP_074424072.1	IS5 family transposase	-
CU111_RS26910	9859..11346	+	1488	Protein_13	Mov34/MPN/PAD-1 family protein	-
CU111_RS26915	11393..12541	+	1149	Protein_14	translesion error-prone DNA polymerase V subunit UmuC	-
CU111_RS26920	12642..13085	+	444	WP_023287184.1	DUF2384 domain-containing protein	Antitoxin
CU111_RS26925	13082..13552	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
CU111_RS26930	13639..14634	+	996	WP_107326708.1	IS5 family transposase	-
CU111_RS26935	14636..15604	+	969	WP_072096747.1	IS5 family transposase	-
CU111_RS26940	15793..16053	-	261	WP_017901409.1	hypothetical protein	-
CU111_RS26945	16358..17326	+	969	WP_074423483.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D) / catA1 / dfrA5 / qacE	-	1..227692	227692
	-	inside	IScluster/Tn	-	-	5914..17326	11412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T89024 WP_004144067.1 NZ_CP024875:13082-13552 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T89024 NZ_CP024875:13082-13552 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16522.84 Da
• Isoelectric Point: 10.3013

>AT89024 WP_023287184.1 NZ_CP024875:12642-13085 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT89024 NZ_CP024875:12642-13085 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6A8PHD0