Detailed information of TA system TA089017

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3476523..3477295
• Replicon: chromosome
• Accession: NZ_CP024874
• Organism: Klebsiella pneumoniae strain NH25

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8B4TZ74
• Locus tag: CU111_RS17315
• Protein ID: WP_044347594.1
• Coordinates: 3476523..3476912 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CU111_RS17320
• Protein ID: WP_177339494.1
• Coordinates: 3476969..3477295 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU111_RS30515	3471931..3472179	+	249	WP_129073712.1	hypothetical protein	-
CU111_RS17290	3472176..3472815	+	640	Protein_3296	RES domain-containing protein	-
CU111_RS17295	3473014..3473202	-	189	Protein_3297	transposase	-
CU111_RS17300	3473219..3474330	-	1112	Protein_3298	IS3 family transposase	-
CU111_RS17305	3474644..3475441	-	798	WP_044347598.1	helix-turn-helix transcriptional regulator	-
CU111_RS17310	3475591..3476436	-	846	WP_044347596.1	DUF4942 domain-containing protein	-
CU111_RS17315	3476523..3476912	-	390	WP_044347594.1	TA system toxin CbtA family protein	Toxin
CU111_RS17320	3476969..3477295	-	327	WP_177339494.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CU111_RS17325	3477353..3477574	-	222	WP_044347590.1	DUF987 domain-containing protein	-
CU111_RS17330	3477588..3478067	-	480	WP_044347588.1	DNA repair protein RadC	-
CU111_RS17335	3478079..3478522	-	444	WP_014837277.1	antirestriction protein	-
CU111_RS17340	3478602..3478850	-	249	WP_044347584.1	DUF905 domain-containing protein	-
CU111_RS17345	3478856..3479680	-	825	WP_040217125.1	DUF945 domain-containing protein	-
CU111_RS17350	3480222..3480854	+	633	WP_072040125.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3464094..3490338	26244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14386.44 Da
• Isoelectric Point: 9.8627

>T89017 WP_044347594.1 NZ_CP024874:c3476912-3476523 [Klebsiella pneumoniae]
MQTKSSPLMRAASSRPSPVYIWQTLLTSLLANHYGLTLSDTPFSDEQVIQQHIDAGISLADALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKAAGLMNRDTYKEVTAITRGQHPQVNTSGKR

Nucleotide

• Length: 390 bp

>T89017 NZ_CP024874:c3476912-3476523 [Klebsiella pneumoniae]
ATGCAAACCAAATCATCCCCCCTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGTATATCTGGCAGACCCTGCTGAC
CAGCCTGCTGGCAAACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAGCAGGTCATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGCCGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAGCGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCCGAGGGCAACATCCGCAGGTTAACACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12126.78 Da
• Isoelectric Point: 6.3156

>AT89017 WP_177339494.1 NZ_CP024874:c3477295-3476969 [Klebsiella pneumoniae]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLDDRANFDGQFCDADLRHLDQAFPVLLKQLELMLTSGELNPRHQHGVTLYA
KGLICEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT89017 NZ_CP024874:c3477295-3476969 [Klebsiella pneumoniae]
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGTGCCCGACTGGTCCAGGAGGGAAACCGCCT
GCATTATCTGGATGACCGGGCGAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGCTGAAACAGCTGGAGTTAATGCTTACCAGCGGTGAGCTGAATCCCCGTCATCAGCACGGCGTCACATTGTATGCA
AAAGGTCTGATCTGCGAGGCGGATACGCTCGGTTCGCATGGCTATGTTTATATCGCGATTTATCCCACTCCCGTCGCCAC
GGCATAA

Structures

Toxin

• AlphaFold DB: A0A8B4TZ74