Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2802819..2803438 Replicon chromosome
Accession NZ_CP024874
Organism Klebsiella pneumoniae strain NH25

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CU111_RS14030 Protein ID WP_002892050.1
Coordinates 2803220..2803438 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CU111_RS14025 Protein ID WP_002892066.1
Coordinates 2802819..2803193 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU111_RS14015 2797971..2799164 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CU111_RS14020 2799187..2802333 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CU111_RS14025 2802819..2803193 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CU111_RS14030 2803220..2803438 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CU111_RS14035 2803597..2804163 + 567 WP_002892030.1 maltose O-acetyltransferase -
CU111_RS30930 2804135..2804275 - 141 WP_004147370.1 hypothetical protein -
CU111_RS14040 2804296..2804766 + 471 WP_002892026.1 YlaC family protein -
CU111_RS14045 2804741..2806192 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CU111_RS14050 2806293..2806991 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
CU111_RS14055 2806988..2807128 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CU111_RS14060 2807128..2807391 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T89015 WP_002892050.1 NZ_CP024874:2803220-2803438 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T89015 NZ_CP024874:2803220-2803438 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT89015 WP_002892066.1 NZ_CP024874:2802819-2803193 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT89015 NZ_CP024874:2802819-2803193 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References