Detailed information of TA system TA088984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1601..2226
• Replicon: plasmid unitig_2_pilon
• Accession: NZ_CP024864
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM342_RS27300
• Protein ID: WP_100190808.1
• Coordinates: 1601..1999 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D6IDB0
• Locus tag: AM342_RS27305
• Protein ID: WP_000450523.1
• Coordinates: 1999..2226 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS27290	381..1074	+	694	Protein_1	hypothetical protein	-
AM342_RS27295	1081..1584	+	504	WP_100190807.1	DUF2726 domain-containing protein	-
AM342_RS27300	1601..1999	-	399	WP_100190808.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AM342_RS27305	1999..2226	-	228	WP_000450523.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AM342_RS27310	2359..4545	+	2187	WP_100190809.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / blaTEM-210	-	1..88768	88768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14914.30 Da
• Isoelectric Point: 8.5187

>T88984 WP_100190808.1 NZ_CP024864:c1999-1601 [Escherichia coli]
MLKFMLDTNICIFTIKNKPAHIRERFNLNTGRMCISSVTVMELVYGAEKSQFPERNLAIIEGFISRLDVLDYDCAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERIAGLRLEGWC

Nucleotide

• Length: 399 bp

>T88984 NZ_CP024864:c1999-1601 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACCATCAAAAATAAACCAGCGCATATCCGGGAGCGTTTCAA
TCTGAATACAGGGCGAATGTGCATCAGCTCAGTGACCGTGATGGAGCTGGTATACGGTGCTGAAAAAAGTCAGTTTCCGG
AGCGTAATCTCGCGATTATCGAAGGATTTATTTCGCGCCTTGACGTGCTGGATTACGACTGCGCTGCTGCCACACATACC
GGTCAGATCCGTGCTGAGCTCGCCCGTCAGGGACGTCCGGTGGGACCATTTGACCAGATGATTGCCGGTCATGCCCGCAG
CCGGGGACTCATTGTGGTCACAAACAATACCCGGGAGTTTGAACGTATCGCCGGCCTTCGTCTGGAAGGCTGGTGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8398.39 Da
• Isoelectric Point: 4.3719

>AT88984 WP_000450523.1 NZ_CP024864:c2226-1999 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVDVIAVGRTRIITPAGESWDSWFDGDSVSADFMNDREQPAVQERESF

Nucleotide

• Length: 228 bp

>AT88984 NZ_CP024864:c2226-1999 [Escherichia coli]
ATGGAAACGACCGTATTTCTCAGTAACCGGAGTCAGGCAGTCAGGCTGCCGAAAGCTGTAGCCCTGCCTGAAGACGTAAA
AAAAGTCGACGTTATTGCTGTTGGCCGTACCCGGATCATTACCCCGGCAGGAGAGTCCTGGGACAGCTGGTTCGATGGCG
ACAGTGTCAGCGCAGATTTCATGAATGACAGAGAACAACCCGCCGTACAGGAAAGGGAGTCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7L6LFS1