Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36080..36605 Replicon plasmid unitig_1_pilon
Accession NZ_CP024863
Organism Escherichia coli strain AR_0015

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AM342_RS26585 Protein ID WP_001159868.1
Coordinates 36080..36385 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag AM342_RS26590 Protein ID WP_023909027.1
Coordinates 36387..36605 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM342_RS26570 31990..33156 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
AM342_RS26575 33744..34499 - 756 WP_000852146.1 replication initiation protein RepE -
AM342_RS26580 35273..36079 - 807 WP_000016982.1 site-specific integrase -
AM342_RS26585 36080..36385 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AM342_RS26590 36387..36605 - 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM342_RS26595 37239..37436 + 198 WP_000215657.1 hypothetical protein -
AM342_RS26600 37433..37717 - 285 WP_000642771.1 hypothetical protein -
AM342_RS26605 37737..38870 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) / dfrA17 / aadA5 / qacE / sul1 / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aac(3)-IId - 1..139059 139059


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T88977 WP_001159868.1 NZ_CP024863:c36385-36080 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T88977 NZ_CP024863:c36385-36080 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT88977 WP_023909027.1 NZ_CP024863:c36605-36387 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT88977 NZ_CP024863:c36605-36387 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References