Detailed information of TA system TA088963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4229965..4230670
• Replicon: chromosome
• Accession: NZ_CP024862
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: AM342_RS21465
• Protein ID: WP_000539521.1
• Coordinates: 4230284..4230670 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AM342_RS21460
• Protein ID: WP_001280945.1
• Coordinates: 4229965..4230294 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS21445	4225122..4226033	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
AM342_RS21450	4226211..4228559	+	2349	WP_021523051.1	EAL domain-containing protein	-
AM342_RS21455	4228567..4229895	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
AM342_RS21460	4229965..4230294	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
AM342_RS21465	4230284..4230670	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM342_RS21470	4230896..4232221	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
AM342_RS21475	4232434..4232817	+	384	WP_000497137.1	biofilm formation regulator BssR	-
AM342_RS21480	4232928..4234043	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
AM342_RS21485	4234040..4234666	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T88963 WP_000539521.1 NZ_CP024862:c4230670-4230284 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T88963 NZ_CP024862:c4230670-4230284 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT88963 WP_001280945.1 NZ_CP024862:c4230294-4229965 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT88963 NZ_CP024862:c4230294-4229965 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6