Detailed information of TA system TA088959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3365483..3365970
• Replicon: chromosome
• Accession: NZ_CP024862
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: AM342_RS17280
• Protein ID: WP_001520461.1
• Coordinates: 3365719..3365970 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: AM342_RS17275
• Protein ID: WP_001520458.1
• Coordinates: 3365483..3365716 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS17250	3360557..3360844	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
AM342_RS17255	3360903..3362234	+	1332	WP_001520455.1	MFS transporter	-
AM342_RS17260	3362342..3362872	+	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
AM342_RS17265	3362865..3364727	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
AM342_RS17270	3364918..3365397	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
AM342_RS17275	3365483..3365716	+	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AM342_RS17280	3365719..3365970	+	252	WP_001520461.1	CcdB family protein	Toxin
AM342_RS17285	3366030..3366878	-	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
AM342_RS17290	3366885..3367262	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
AM342_RS17295	3367265..3368086	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
AM342_RS17300	3368083..3369072	-	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
AM342_RS17305	3369072..3370358	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T88959 WP_001520461.1 NZ_CP024862:3365719-3365970 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T88959 NZ_CP024862:3365719-3365970 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT88959 WP_001520458.1 NZ_CP024862:3365483-3365716 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT88959 NZ_CP024862:3365483-3365716 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4