Detailed information of TA system TA088955

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2928511..2929346
• Replicon: chromosome
• Accession: NZ_CP024862
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J1YPH7
• Locus tag: AM342_RS14845
• Protein ID: WP_029701676.1
• Coordinates: 2928511..2928888 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: AM342_RS14850
• Protein ID: WP_021553056.1
• Coordinates: 2928978..2929346 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS14820	2924878..2926416	-	1539	WP_100190781.1	lysine decarboxylation/transport transcriptional activator CadC	-
AM342_RS14830	2927165..2928007	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
AM342_RS14835	2928092..2928286	-	195	WP_024181941.1	DUF957 domain-containing protein	-
AM342_RS28330	2928365..2928514	-	150	Protein_2701	hypothetical protein	-
AM342_RS14845	2928511..2928888	-	378	WP_029701676.1	TA system toxin CbtA family protein	Toxin
AM342_RS14850	2928978..2929346	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM342_RS14855	2929509..2929730	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM342_RS14860	2929795..2930271	-	477	WP_021553055.1	RadC family protein	-
AM342_RS14865	2930287..2930766	-	480	WP_001564060.1	antirestriction protein	-
AM342_RS14870	2931032..2931850	-	819	WP_001175165.1	DUF945 domain-containing protein	-
AM342_RS14875	2931940..2932173	-	234	WP_001278293.1	DUF905 family protein	-
AM342_RS14880	2932179..2932856	-	678	WP_001097302.1	hypothetical protein	-
AM342_RS14885	2933004..2933684	-	681	WP_029702103.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2917390..2963149	45759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14066.04 Da
• Isoelectric Point: 7.8045

>T88955 WP_029701676.1 NZ_CP024862:c2928888-2928511 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T88955 NZ_CP024862:c2928888-2928511 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT88955 WP_021553056.1 NZ_CP024862:c2929346-2928978 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT88955 NZ_CP024862:c2929346-2928978 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J1YPH7

Antitoxin

• AlphaFold DB: A0A0K5N986