Detailed information of TA system TA088937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 68646..69172
• Replicon: chromosome
• Accession: NZ_CP024862
• Organism: Escherichia coli strain AR_0015

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AM342_RS00350
• Protein ID: WP_000323025.1
• Coordinates: 68646..68933 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AM342_RS00355
• Protein ID: WP_000534858.1
• Coordinates: 68933..69172 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM342_RS00305	63730..64188	-	459	WP_000526135.1	IS200/IS605 family transposase	-
AM342_RS00315	64639..64854	-	216	WP_000066484.1	cold shock-like protein CspB	-
AM342_RS00320	65155..65367	+	213	WP_000087756.1	cold shock-like protein CspF	-
AM342_RS28190	65422..65511	+	90	WP_120795389.1	hypothetical protein	-
AM342_RS00325	65789..66541	-	753	WP_001047135.1	antitermination protein	-
AM342_RS00330	66555..67604	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
AM342_RS00335	67606..67884	-	279	WP_012304870.1	hypothetical protein	-
AM342_RS00340	67951..68202	-	252	WP_000980994.1	hypothetical protein	-
AM342_RS00345	68419..68574	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
AM342_RS00350	68646..68933	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AM342_RS00355	68933..69172	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AM342_RS00360	69197..69502	+	306	WP_001326990.1	hypothetical protein	-
AM342_RS00365	69705..70037	+	333	WP_001301033.1	protein FlxA	-
AM342_RS00375	70474..71787	-	1314	WP_000589005.1	ISNCY family transposase	-
AM342_RS00380	72271..73299	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
AM342_RS00385	73296..73910	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	35753..86397	50644
	-	flank	IS/Tn	-	-	63730..64188	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T88937 WP_000323025.1 NZ_CP024862:c68933-68646 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T88937 NZ_CP024862:c68933-68646 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT88937 WP_000534858.1 NZ_CP024862:c69172-68933 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT88937 NZ_CP024862:c69172-68933 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8