88935

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 12593..13058 Replicon plasmid unitig_2_pilon
Accession NZ_CP024861
Organism Escherichia coli strain AR_0014

Toxin (Protein)


Gene name TsxA Uniprot ID M7C8T2
Locus tag AM341_RS25950 Protein ID WP_001230706.1
Coordinates 12593..12841 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag AM341_RS25955 Protein ID WP_000801440.1
Coordinates 12831..13058 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM341_RS25920 7723..8127 - 405 WP_198432272.1 hypothetical protein -
AM341_RS25925 8140..9006 - 867 WP_001353743.1 replication initiation protein -
AM341_RS25930 10112..10447 + 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -
AM341_RS25935 10444..10794 + 351 WP_000127482.1 addiction module antitoxin -
AM341_RS26485 11434..11949 + 516 WP_001025395.1 J domain-containing protein -
AM341_RS25945 12054..12596 + 543 WP_001083906.1 hypothetical protein -
AM341_RS25950 12593..12841 + 249 WP_001230706.1 hypothetical protein Toxin
AM341_RS25955 12831..13058 + 228 WP_000801440.1 hypothetical protein Antitoxin
AM341_RS25965 13512..13811 + 300 WP_000650311.1 hypothetical protein -
AM341_RS25970 13821..14237 + 417 WP_012421208.1 tail-associated lysozyme -
AM341_RS25975 14234..14545 + 312 WP_000627398.1 hypothetical protein -
AM341_RS25980 14855..15103 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
AM341_RS25985 15100..15447 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
AM341_RS25995 15810..16313 + 504 WP_000781814.1 hypothetical protein -
AM341_RS26000 16317..17546 + 1230 WP_016231858.1 relaxase/mobilization nuclease domain-containing protein -
AM341_RS26005 17623..17916 + 294 WP_000134733.1 LuxR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33548 33548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type I toxin-antitoxin system, toxin Ldr

(1-35)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9594.94 Da        Isoelectric Point: 7.9925

>T88935 WP_001230706.1 NZ_CP024861:12593-12841 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ET

Download         Length: 249 bp

>T88935 NZ_CP024861:12593-12841 [Escherichia coli]
ATGAGAAAAAATAAAGGCGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAAAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT88935 WP_000801440.1 NZ_CP024861:12831-13058 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT88935 NZ_CP024861:12831-13058 [Escherichia coli]
ATGAAACCTGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M7C8T2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References