Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3552965..3553619 Replicon chromosome
Accession NZ_CP024859
Organism Escherichia coli strain AR_0014

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AM341_RS18360 Protein ID WP_000244777.1
Coordinates 3553212..3553619 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AM341_RS18355 Protein ID WP_000354046.1
Coordinates 3552965..3553231 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM341_RS18330 3548134..3548877 + 744 WP_000951964.1 SDR family oxidoreductase -
AM341_RS18335 3548934..3550367 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
AM341_RS18340 3550412..3550723 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
AM341_RS18345 3550887..3551546 + 660 WP_000250274.1 hemolysin III family protein -
AM341_RS18350 3551742..3552722 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AM341_RS18355 3552965..3553231 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AM341_RS18360 3553212..3553619 + 408 WP_000244777.1 toxin CptA Toxin
AM341_RS18365 3553659..3554180 - 522 WP_001055874.1 flavodoxin FldB -
AM341_RS18370 3554292..3555188 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AM341_RS18375 3555213..3555923 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AM341_RS18380 3555929..3557662 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T88913 WP_000244777.1 NZ_CP024859:3553212-3553619 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T88913 NZ_CP024859:3553212-3553619 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT88913 WP_000354046.1 NZ_CP024859:3552965-3553231 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT88913 NZ_CP024859:3552965-3553231 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References