Detailed information of TA system TA088903

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1479044..1479738
• Replicon: chromosome
• Accession: NZ_CP024859
• Organism: Escherichia coli strain AR_0014

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: AM341_RS07625
• Protein ID: WP_001094398.1
• Coordinates: 1479044..1479412 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM341_RS07630
• Protein ID: WP_077249034.1
• Coordinates: 1479433..1479738 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM341_RS07605	1474217..1475173	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
AM341_RS07610	1475170..1477368	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM341_RS07615	1477378..1478334	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM341_RS07620	1478385..1478723	+	339	Protein_1381	LysR family transcriptional regulator	-
AM341_RS07625	1479044..1479412	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
AM341_RS07630	1479433..1479738	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM341_RS07635	1479842..1480486	-	645	WP_000086755.1	hypothetical protein	-
AM341_RS07640	1480505..1480726	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AM341_RS07645	1480795..1481271	-	477	WP_001424026.1	RadC family protein	-
AM341_RS07650	1481287..1481760	-	474	WP_000855059.1	antirestriction protein	-
AM341_RS07660	1482102..1482923	-	822	WP_001234565.1	DUF945 domain-containing protein	-
AM341_RS26250	1483024..1483232	-	209	Protein_1389	DUF905 domain-containing protein	-
AM341_RS07670	1483333..1483788	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	1451489..1493387	41898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T88903 WP_001094398.1 NZ_CP024859:c1479412-1479044 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T88903 NZ_CP024859:c1479412-1479044 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT88903 WP_077249034.1 NZ_CP024859:c1479738-1479433 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT88903 NZ_CP024859:c1479738-1479433 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4