Detailed information of TA system TA088899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 14886..15387
• Replicon: plasmid tig00013784_pilon
• Accession: NZ_CP024858
• Organism: Escherichia coli strain AR_0011

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: AM338_RS26550
• Protein ID: WP_000702246.1
• Coordinates: 15127..15387 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: AM338_RS26545
• Protein ID: WP_001132407.1
• Coordinates: 14886..15137 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM338_RS26495	10081..10782	+	702	WP_000280995.1	hypothetical protein	-
AM338_RS26500	10829..11428	+	600	WP_000091422.1	hypothetical protein	-
AM338_RS26505	11448..11774	+	327	WP_000206697.1	hypothetical protein	-
AM338_RS26510	11905..12342	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
AM338_RS26515	12367..12549	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
AM338_RS26520	13083..13265	+	183	WP_001481829.1	hypothetical protein	-
AM338_RS26525	13372..13638	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
AM338_RS26530	14058..14336	-	279	WP_000545937.1	hypothetical protein	-
AM338_RS26545	14886..15137	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
AM338_RS26550	15127..15387	-	261	WP_000702246.1	hypothetical protein	Toxin
AM338_RS26555	15438..15662	-	225	WP_000814384.1	hypothetical protein	-
AM338_RS26560	15760..15969	-	210	WP_000866037.1	hypothetical protein	-
AM338_RS26565	16011..16334	-	324	WP_000427402.1	hypothetical protein	-
AM338_RS26570	16915..17244	-	330	WP_000855118.1	hypothetical protein	-
AM338_RS26580	17842..18507	+	666	WP_000532148.1	P-loop NTPase	-
AM338_RS27430	18586..18870	+	285	WP_001162371.1	hypothetical protein	-
AM338_RS26590	18888..19484	-	597	WP_000125165.1	hypothetical protein	-
AM338_RS26595	19634..19795	-	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
AM338_RS27435	19988..20149	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..57991	57991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T88899 WP_000702246.1 NZ_CP024858:c15387-15127 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T88899 NZ_CP024858:c15387-15127 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT88899 WP_001132407.1 NZ_CP024858:c15137-14886 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT88899 NZ_CP024858:c15137-14886 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7