Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30170..30813 Replicon plasmid tig00001069_pilon
Accession NZ_CP024857
Organism Escherichia coli strain AR_0011

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag AM338_RS26160 Protein ID WP_000754566.1
Coordinates 30170..30586 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag AM338_RS26165 Protein ID WP_001261276.1
Coordinates 30583..30813 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM338_RS26125 25187..25465 - 279 Protein_16 IS3 family transposase -
AM338_RS26130 25482..26558 - 1077 WP_000227969.1 IS110 family transposase -
AM338_RS26140 26840..27019 - 180 Protein_18 DDE-type integrase/transposase/recombinase -
AM338_RS26145 27100..28608 - 1509 WP_001189123.1 group II intron reverse transcriptase/maturase -
AM338_RS26155 29281..29966 - 686 Protein_20 IS3-like element ISEc15 family transposase -
AM338_RS26160 30170..30586 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
AM338_RS26165 30583..30813 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM338_RS26175 31387..31737 + 351 WP_000493378.1 hypothetical protein -
AM338_RS26180 31788..32531 + 744 WP_000129823.1 hypothetical protein -
AM338_RS26185 32528..33304 + 777 WP_000015958.1 site-specific integrase -
AM338_RS26190 33362..33619 - 258 WP_000764642.1 hypothetical protein -
AM338_RS27420 33748..33852 - 105 WP_032409716.1 hypothetical protein -
AM338_RS26195 34382..35248 + 867 WP_004118283.1 replication initiation protein -
AM338_RS26200 35425..35694 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 - 1..76680 76680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T88898 WP_000754566.1 NZ_CP024857:c30586-30170 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T88898 NZ_CP024857:c30586-30170 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT88898 WP_001261276.1 NZ_CP024857:c30813-30583 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT88898 NZ_CP024857:c30813-30583 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References