Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 45506..46031 Replicon plasmid tig00001011_pilon
Accession NZ_CP024856
Organism Escherichia coli strain AR_0011

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AM338_RS25180 Protein ID WP_001159871.1
Coordinates 45506..45811 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag AM338_RS25185 Protein ID WP_000813630.1
Coordinates 45813..46031 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM338_RS25165 41468..42634 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
AM338_RS25170 43222..43977 - 756 WP_000852146.1 replication initiation protein RepE -
AM338_RS25175 44699..45505 - 807 WP_000016970.1 site-specific integrase -
AM338_RS25180 45506..45811 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AM338_RS25185 45813..46031 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM338_RS25195 46591..46821 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AM338_RS25200 46818..47234 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
AM338_RS25205 47309..48874 + 1566 WP_001128474.1 AAA family ATPase -
AM338_RS25210 48859..49881 + 1023 WP_000361402.1 helicase UvrD -
AM338_RS25220 50135..50821 - 687 Protein_66 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / tet(A) iutA / iucD / iucC / iucB / iucA / senB 1..181436 181436
- flank IS/Tn - - 50135..50482 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T88894 WP_001159871.1 NZ_CP024856:c45811-45506 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T88894 NZ_CP024856:c45811-45506 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT88894 WP_000813630.1 NZ_CP024856:c46031-45813 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT88894 NZ_CP024856:c46031-45813 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References