Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4772927..4773545 Replicon chromosome
Accession NZ_CP024855
Organism Escherichia coli strain AR_0011

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM338_RS24450 Protein ID WP_001291435.1
Coordinates 4772927..4773145 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM338_RS24455 Protein ID WP_000344800.1
Coordinates 4773171..4773545 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM338_RS24415 4768216..4768788 + 573 WP_000779831.1 YbaY family lipoprotein -
AM338_RS24420 4768819..4769130 - 312 WP_000409911.1 MGMT family protein -
AM338_RS24430 4769509..4769862 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AM338_RS24435 4769904..4771454 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM338_RS24440 4771618..4772088 - 471 WP_000136192.1 YlaC family protein -
AM338_RS24445 4772204..4772755 - 552 WP_000102564.1 maltose O-acetyltransferase -
AM338_RS24450 4772927..4773145 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM338_RS24455 4773171..4773545 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM338_RS24460 4774091..4777240 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
AM338_RS24465 4777263..4778456 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88889 WP_001291435.1 NZ_CP024855:c4773145-4772927 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T88889 NZ_CP024855:c4773145-4772927 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCTGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT88889 WP_000344800.1 NZ_CP024855:c4773545-4773171 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT88889 NZ_CP024855:c4773545-4773171 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References