Detailed information of TA system TA088888

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4587473..4588167
• Replicon: chromosome
• Accession: NZ_CP024855
• Organism: Escherichia coli strain AR_0011

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: AM338_RS23485
• Protein ID: WP_001094398.1
• Coordinates: 4587799..4588167 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM338_RS23480
• Protein ID: WP_077249034.1
• Coordinates: 4587473..4587778 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM338_RS23440	4583423..4583878	+	456	WP_000581506.1	hypothetical protein	-
AM338_RS27135	4583979..4584187	+	209	Protein_4280	DUF905 domain-containing protein	-
AM338_RS23450	4584288..4585109	+	822	WP_001234565.1	DUF945 domain-containing protein	-
AM338_RS23460	4585451..4585924	+	474	WP_000855059.1	antirestriction protein	-
AM338_RS23465	4585940..4586416	+	477	WP_001424026.1	RadC family protein	-
AM338_RS23470	4586485..4586706	+	222	WP_000692345.1	DUF987 domain-containing protein	-
AM338_RS23475	4586725..4587369	+	645	WP_000086755.1	hypothetical protein	-
AM338_RS23480	4587473..4587778	+	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM338_RS23485	4587799..4588167	+	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
AM338_RS23490	4588488..4588826	-	339	Protein_4288	LysR family transcriptional regulator	-
AM338_RS23495	4588877..4589833	-	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
AM338_RS23500	4589843..4592041	-	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
AM338_RS23505	4592038..4592994	-	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4571814..4594712	22898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T88888 WP_001094398.1 NZ_CP024855:4587799-4588167 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T88888 NZ_CP024855:4587799-4588167 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT88888 WP_077249034.1 NZ_CP024855:4587473-4587778 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT88888 NZ_CP024855:4587473-4587778 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4