Detailed information of TA system TA088840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3552006..3552532
• Replicon: chromosome
• Accession: NZ_CP024851
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: AM333_RS18360
• Protein ID: WP_000323025.1
• Coordinates: 3552006..3552293 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: AM333_RS18365
• Protein ID: WP_000534858.1
• Coordinates: 3552293..3552532 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS18315	3547090..3547548	-	459	WP_000526135.1	IS200/IS605 family transposase	-
AM333_RS18325	3547999..3548214	-	216	WP_000066484.1	cold shock-like protein CspB	-
AM333_RS18330	3548515..3548727	+	213	WP_000087756.1	cold shock-like protein CspF	-
AM333_RS28120	3548782..3548871	+	90	WP_120795389.1	hypothetical protein	-
AM333_RS18335	3549149..3549901	-	753	WP_001047135.1	antitermination protein	-
AM333_RS18340	3549915..3550964	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
AM333_RS18345	3550966..3551244	-	279	WP_012304870.1	hypothetical protein	-
AM333_RS18350	3551311..3551562	-	252	WP_000980994.1	hypothetical protein	-
AM333_RS18355	3551779..3551934	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
AM333_RS18360	3552006..3552293	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
AM333_RS18365	3552293..3552532	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
AM333_RS18370	3552557..3552862	+	306	WP_001326990.1	hypothetical protein	-
AM333_RS18375	3553065..3553397	+	333	WP_001301033.1	protein FlxA	-
AM333_RS18385	3553834..3555147	-	1314	WP_000589005.1	ISNCY family transposase	-
AM333_RS18390	3555631..3556659	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
AM333_RS18395	3556656..3557270	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3515687..3569757	54070
	-	flank	IS/Tn	-	-	3547090..3547548	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T88840 WP_000323025.1 NZ_CP024851:c3552293-3552006 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T88840 NZ_CP024851:c3552293-3552006 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT88840 WP_000534858.1 NZ_CP024851:c3552532-3552293 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT88840 NZ_CP024851:c3552532-3552293 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8