Detailed information of TA system TA088838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3371941..3372579
• Replicon: chromosome
• Accession: NZ_CP024851
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: AM333_RS17420
• Protein ID: WP_001306887.1
• Coordinates: 3371941..3372117 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM333_RS17425
• Protein ID: WP_001270286.1
• Coordinates: 3372163..3372579 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS17395	3366997..3367455	+	459	WP_000526135.1	IS200/IS605 family transposase	-
AM333_RS17400	3367563..3368774	-	1212	WP_023143671.1	BenE family transporter YdcO	-
AM333_RS17405	3368827..3369363	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
AM333_RS17410	3369436..3371397	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AM333_RS17415	3371489..3371719	-	231	WP_000494241.1	YncJ family protein	-
AM333_RS17420	3371941..3372117	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AM333_RS17425	3372163..3372579	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AM333_RS17430	3372658..3374064	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
AM333_RS17435	3374309..3375454	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
AM333_RS17440	3375472..3376485	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
AM333_RS17445	3376486..3377427	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3366997..3367455	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T88838 WP_001306887.1 NZ_CP024851:3371941-3372117 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T88838 NZ_CP024851:3371941-3372117 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT88838 WP_001270286.1 NZ_CP024851:3372163-3372579 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT88838 NZ_CP024851:3372163-3372579 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76