Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2223942..2224560 Replicon chromosome
Accession NZ_CP024851
Organism Escherichia coli strain AR_0006

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM333_RS11450 Protein ID WP_001291435.1
Coordinates 2223942..2224160 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM333_RS11455 Protein ID WP_000344800.1
Coordinates 2224186..2224560 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM333_RS11415 2219231..2219803 + 573 WP_000779831.1 YbaY family lipoprotein -
AM333_RS11420 2219834..2220145 - 312 WP_000409911.1 MGMT family protein -
AM333_RS11430 2220524..2220877 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AM333_RS11435 2220919..2222469 - 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM333_RS11440 2222633..2223103 - 471 WP_001490888.1 YlaC family protein -
AM333_RS11445 2223219..2223770 - 552 WP_001522034.1 maltose O-acetyltransferase -
AM333_RS11450 2223942..2224160 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM333_RS11455 2224186..2224560 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM333_RS11460 2225106..2228255 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
AM333_RS11465 2228278..2229471 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T88829 WP_001291435.1 NZ_CP024851:c2224160-2223942 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T88829 NZ_CP024851:c2224160-2223942 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT88829 WP_000344800.1 NZ_CP024851:c2224560-2224186 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT88829 NZ_CP024851:c2224560-2224186 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References