Detailed information of TA system TA088825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1614060..1614874
• Replicon: chromosome
• Accession: NZ_CP024851
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: AM333_RS08415
• Protein ID: WP_001054376.1
• Coordinates: 1614617..1614874 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: AM333_RS08410
• Protein ID: WP_001309181.1
• Coordinates: 1614060..1614605 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS08385	1609751..1611064	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
AM333_RS08390	1611076..1611354	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
AM333_RS08395	1611351..1612472	-	1122	WP_000010829.1	M42 family metallopeptidase	-
AM333_RS28270	1612717..1612833	-	117	Protein_1489	VOC family protein	-
AM333_RS08400	1612871..1613089	-	219	Protein_1490	hypothetical protein	-
AM333_RS08405	1613258..1614004	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
AM333_RS08410	1614060..1614605	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
AM333_RS08415	1614617..1614874	-	258	WP_001054376.1	hypothetical protein	Toxin
AM333_RS08425	1615251..1615496	-	246	Protein_1494	GNAT family N-acetyltransferase	-
AM333_RS08430	1615760..1617352	-	1593	WP_048237919.1	IS66 family transposase	-
AM333_RS08435	1617383..1617733	-	351	WP_000624718.1	IS66 family insertion sequence element accessory protein TnpB	-
AM333_RS08440	1617730..1618155	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
AM333_RS08450	1618242..1619371	+	1130	Protein_1498	helicase YjhR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T88825 WP_001054376.1 NZ_CP024851:c1614874-1614617 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T88825 NZ_CP024851:c1614874-1614617 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT88825 WP_001309181.1 NZ_CP024851:c1614605-1614060 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT88825 NZ_CP024851:c1614605-1614060 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8