Detailed information of TA system TA088823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1501178..1501773
• Replicon: chromosome
• Accession: NZ_CP024851
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: AM333_RS07755
• Protein ID: WP_000239581.1
• Coordinates: 1501423..1501773 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: AM333_RS07750
• Protein ID: WP_001223213.1
• Coordinates: 1501178..1501429 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS07740	1496843..1500622	+	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-
AM333_RS07745	1500625..1500966	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
AM333_RS07750	1501178..1501429	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
AM333_RS07755	1501423..1501773	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
AM333_RS07760	1501852..1502382	-	531	WP_000055075.1	inorganic diphosphatase	-
AM333_RS07765	1502692..1503648	+	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
AM333_RS07770	1503781..1505283	+	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
AM333_RS07775	1505297..1506319	+	1023	WP_001361374.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T88823 WP_000239581.1 NZ_CP024851:1501423-1501773 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T88823 NZ_CP024851:1501423-1501773 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT88823 WP_001223213.1 NZ_CP024851:1501178-1501429 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT88823 NZ_CP024851:1501178-1501429 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7