Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 4784..5921 Replicon plasmid unnamed1
Accession NZ_CP024844
Organism Streptococcus iniae strain FP5228

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag CTW00_RS10190 Protein ID WP_100185210.1
Coordinates 5058..5921 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag CTW00_RS10185 Protein ID WP_000301765.1
Coordinates 4784..5056 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTW00_RS10340 509..679 + 171 WP_000713595.1 hypothetical protein -
CTW00_RS10165 693..1310 + 618 WP_100185207.1 recombinase family protein -
CTW00_RS10170 1310..3454 + 2145 WP_100185208.1 type IA DNA topoisomerase -
CTW00_RS10175 3557..4453 + 897 WP_002387620.1 ParA family protein -
CTW00_RS10180 4558..4767 + 210 WP_000527317.1 peptide-binding protein -
CTW00_RS10185 4784..5056 + 273 WP_000301765.1 antitoxin Antitoxin
CTW00_RS10190 5058..5921 + 864 WP_100185210.1 zeta toxin family protein Toxin
CTW00_RS10195 6357..6674 + 318 WP_002338433.1 hypothetical protein -
CTW00_RS10200 6694..7191 + 498 WP_010713926.1 molecular chaperone DnaJ -
CTW00_RS10205 7225..7458 + 234 WP_085444310.1 thymidylate synthase ThyX -
CTW00_RS10210 7486..8166 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
CTW00_RS10215 8413..9060 - 648 WP_002352254.1 type A-8 chloramphenicol O-acetyltransferase -
CTW00_RS10220 9195..9506 - 312 Protein_13 replication initiation protein -
CTW00_RS10225 9704..10384 + 681 WP_172952525.1 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid cat / erm(B) - 1..13781 13781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32416.95 Da        Isoelectric Point: 6.6610

>T88814 WP_100185210.1 NZ_CP024844:5058-5921 [Streptococcus iniae]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T88814 NZ_CP024844:5058-5921 [Streptococcus iniae]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTACGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT88814 WP_000301765.1 NZ_CP024844:4784-5056 [Streptococcus iniae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT88814 NZ_CP024844:4784-5056 [Streptococcus iniae]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References