Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 77389..77912 Replicon plasmid pCRKP-1215_1
Accession NZ_CP024839
Organism Klebsiella pneumoniae strain CRKP-1215

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag CU074_RS27750 Protein ID WP_015632464.1
Coordinates 77607..77912 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag CU074_RS27745 Protein ID WP_015632465.1
Coordinates 77389..77607 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU074_RS27725 73532..74803 - 1272 WP_016162068.1 Y-family DNA polymerase -
CU074_RS27730 74803..75234 - 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
CU074_RS27735 75398..75883 - 486 Protein_79 IS5 family transposase -
CU074_RS27740 76178..77203 - 1026 WP_015632466.1 IS110 family transposase -
CU074_RS27745 77389..77607 + 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CU074_RS27750 77607..77912 + 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
CU074_RS27755 78057..78401 + 345 WP_015632463.1 hypothetical protein -
CU074_RS27760 78443..79009 + 567 WP_015632462.1 hypothetical protein -
CU074_RS27765 79213..79470 - 258 WP_016162071.1 helix-turn-helix domain-containing protein -
CU074_RS27770 79900..80127 + 228 WP_016162072.1 hypothetical protein -
CU074_RS29390 80141..80263 + 123 Protein_87 transcriptional regulator -
CU074_RS27780 80469..80912 - 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
CU074_RS27785 80938..81120 - 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
CU074_RS27790 81481..82461 + 981 WP_015632457.1 partitioning protein -
CU074_RS27795 82454..82882 + 429 WP_015632456.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA2 / dfrA12 / catA2 / rmtF / ARR-3 / qnrB1 - 1..130922 130922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T88788 WP_015632464.1 NZ_CP024839:77607-77912 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T88788 NZ_CP024839:77607-77912 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT88788 WP_015632465.1 NZ_CP024839:77389-77607 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT88788 NZ_CP024839:77389-77607 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References