Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3694416..3695152 Replicon chromosome
Accession NZ_CP024838
Organism Klebsiella pneumoniae strain CRKP-1215

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag CU074_RS18580 Protein ID WP_032433360.1
Coordinates 3694670..3695152 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CU074_RS18575 Protein ID WP_003026799.1
Coordinates 3694416..3694682 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU074_RS18550 3690062..3691201 + 1140 WP_032433368.1 mannitol dehydrogenase -
CU074_RS18555 3691230..3691892 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
CU074_RS18560 3691876..3692880 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
CU074_RS18565 3692898..3693530 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
CU074_RS18570 3693540..3694103 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
CU074_RS18575 3694416..3694682 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CU074_RS18580 3694670..3695152 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
CU074_RS18585 3695513..3696112 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
CU074_RS18590 3696325..3697269 - 945 WP_077254249.1 fimbrial protein -
CU074_RS18595 3697281..3697859 - 579 WP_032432061.1 type 1 fimbrial protein -
CU074_RS18600 3697863..3698603 - 741 WP_100181654.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3664639..3706095 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T88778 WP_032433360.1 NZ_CP024838:3694670-3695152 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T88778 NZ_CP024838:3694670-3695152 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT88778 WP_003026799.1 NZ_CP024838:3694416-3694682 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT88778 NZ_CP024838:3694416-3694682 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References